咖啡酸、Hispidin和一种已发现的活化成分对新南白担子菌菌丝发光及发光系统的影响

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2024-12-20 DOI:10.1134/S0006350924700544
N. O. Ronzhin, E. D. Posokhina, V. M. Le, O. A. Mogilnaya, Yu. V. Zakharova, A. S. Sukhikh, V. S. Bondar
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摘要

研究发现,在发光真菌 Neonothopanus nambi 的菌丝体中加入咖啡酸和我们发现的一种低分子量生物发光激活剂后,其发光强度会迅速显著增加(数量级甚至更高)。据推测,所观察到的真菌发光活化效应可能是由基枝菌木质素分解复合体酶(特别是过氧化物酶)对添加物质的氧化作用以及可见光量子的发射介导的。同时进行的体内实验表明,添加糙硬蛋白(发光高等真菌中荧光素的前体)不会影响菌丝的发光强度。体外研究表明,咖啡酸和已发现的低分子量辉光激活剂在有 NADPH 存在的情况下不影响从 N. nambi 菌丝体中分离出来的酶发光系统的发光水平,但却显著抑制了 NADPH 和糙皮素诱导的该系统的发光反应。我们获得的全部数据表明,在发光的高等真菌中存在不同的生化途径来产生可见光量子,其中涉及不同的酶(或酶系统)和不同的底物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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The Effects of Caffeic Acid, Hispidin and a Discovered Activating Component on Luminescence of Mycelium and a Luminescent System of the Basidiomycete Neonothopanus nambi

It was found in vivo that the addition of caffeic acid and a low-molecular-weight bioluminescence activator that we have discovered to the mycelium of the luminous fungus Neonothopanus nambi led to a rapid and significant (by an order of magnitude and more) increase in the intensity of its light emission. It is proposed that the observed effect of activation of the fungus glow may be mediated by the oxidation of added substances by enzymes of the ligninolytic complex of basidiomycetes (in particular, peroxidases) with the emission of visible light quanta. Parallel in vivo experiments have shown that the addition of hispidin (a precursor of luciferin in luminous higher fungi) did not affect the intensity of light emission of the mycelium. In vitro studies have shown that caffeic acid and the discovered low-molecular-weight glow activator did not affect the light emission level of the enzyme luminescent system isolated from the mycelium of N. nambi in the presence of NADPH and significantly suppressed the luminescent reaction of the system induced by NADPH and hispidin. The totality of the data we obtained indicated the presence in luminous higher fungi of different biochemical pathways for the generation of visible light quanta involving different enzymes (or enzyme systems) and different substrates.

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来源期刊
Biophysics
Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍: Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
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