光动力与等离子体光热联合治疗大鼠移植瘤模型

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2024-12-20 DOI:10.1134/S0006350924700623
A. B. Bucharskaya, N. A. Navolokin, D. A. Mudrak, G. N. Maslyakova, B. N. Khlebtsov, N. G. Khlebtsov, V. D. Genin, E. A. Genina, V. V. Tuchin
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引用次数: 0

摘要

本研究旨在建立等离子体光热和光动力联合治疗大鼠移植胆管癌PC-1的方法。在光动力治疗中,以2mg /kg的剂量,将吲哚菁绿按1:100的比例稀释在聚乙二醇中给鼠瘤内注射。等离子体光热治疗时,用聚乙二醇(400 μg/mL)稀释的金纳米棒按肿瘤体积的30%注射入瘤内。注射后1 h,用波长为808 nm、功率密度为2.3 W/cm2的二极管红外激光器经皮照射肿瘤15 min。治疗后72 h和21 d分别离开实验。在标准染色和免疫组织化学染色的切片上进行肿瘤形态学研究。结合等离子体光热和光动力治疗,肿瘤温度显著升高,高达60.0±4.1°C。72h后,肿瘤组织出现明显的坏死改变,仅在肿瘤周围可见完整的肿瘤细胞。治疗后21天,肿瘤生长明显受到抑制;肿瘤块抑制指数为77.4%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Photodynamic and Plasmonic Photothermal Combination Therapy of Transplanted Tumors of a Rat Model

The aim of this study was to develop a combined technology of plasmon photothermal therapy and photodynamic therapy of transplanted cholangiocarcinoma PC-1 in rats. For photodynamic therapy, rats were intratumorally administered with indocyanine green diluted in polyethylene glycol at a ratio of 1 : 100 at a dose of 2 mg/kg. For plasmon photothermal therapy, gold nanorods diluted in polyethylene glycol (400 μg/mL) were intratumorally injected in a volume of 30% of the tumor volume. At 1 h after injection, the tumor was percutaneously irradiated with a diode infrared laser with a wavelength of 808 nm at a power density of 2.3 W/cm2 for 15 min. The animals were removed from the experiment 72 h and 21 days after therapy. Morphological studies of the tumor were performed on sections stained with standard and immunohistochemical methods. A significant increase in tumor temperature was noted, up to 60.0 ± 4.1°C with combined plasmon photothermal and photodynamic therapy. After 72 h, pronounced necrotic changes were observed in the tumor tissue and intact tumor cells were observed only on the periphery of the tumor. At 21 days after therapy, a significant inhibition of tumor growth was noted; the inhibition index by tumor mass was 77.4%.

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来源期刊
Biophysics
Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍: Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
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