昆虫幼体激素III在植物烟叶中的生物合成工程。

IF 6.8 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Metabolic engineering Pub Date : 2024-12-17 DOI:10.1016/j.ymben.2024.12.005
Angeliki Stathaki, Ryan M Alam, Tobias G Köllner, Sarah E O'Connor
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引用次数: 0

摘要

幼虫激素(JHs)是由法尼松酸衍生的倍半萜类化合物,在调节昆虫的各种发育过程中起着至关重要的作用。基于这些报告的生物活性,JHs及其合成类似物在过去几年中已被用作杀虫剂,并取得了重大的商业成功。本文描述了通过在植物烟叶中的瞬时基因表达来设计黄热病蚊子(埃及伊蚊)的JH途径。该方法成功地在benthamiana叶片中生产了JH III,浓度约为10 μg/g鲜重。拟南芥中3-羟基-3-甲基戊二酰辅酶a还原酶的反馈不敏感版本的共表达进一步将滴度从10提高到80 μg/g鲜重,提高了8倍。我们的研究还揭示了N. benthamiana丰富的内源性代谢背景可以产生法脂酸,这是JH III的关键前体,因此只需要表达3个基因就可以提供高滴度的这种化合物。我们的研究表明,通过昆虫JH合成基因的异源表达,benthamina可以产生高滴度的JH III。
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Engineering of insect juvenile hormone III biosynthesis in the plant Nicotiana benthamiana.

Juvenile hormones (JHs) are farnesoic acid-derived sesquiterpenoids that play a crucial role in regulating various developmental processes in insects. Based on these reported biological activities, JHs and their synthetic analogs have been utilized as insecticides with significant commercial success over the past years. Here we describe the engineering of the JH pathway of the yellow fever mosquito (Aedes aegypti) by transient gene expression in the plant Nicotiana benthamiana. This approach led to the successful production of JH III in N. benthamiana leaves at a concentration of ca. 10 μg/g fresh weight. The co-expression of a feedback-insensitive version of 3-hydroxy-3-methylglutaryl coenzyme A reductase from Arabidopsis thaliana further increased the titer eight-fold from 10 to 80 μg/g fresh weight. Our efforts also revealed that the rich endogenous metabolic background of N. benthamiana can generate farnesoic acid, a key precursor to JH III, and thus, only 3 genes need to be expressed to provide high titers of this compound. Our study demonstrates the production of high titers of JH III in N. benthamina via heterologous expression of insect JH biosynthetic genes.

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来源期刊
Metabolic engineering
Metabolic engineering 工程技术-生物工程与应用微生物
CiteScore
15.60
自引率
6.00%
发文量
140
审稿时长
44 days
期刊介绍: Metabolic Engineering (MBE) is a journal that focuses on publishing original research papers on the directed modulation of metabolic pathways for metabolite overproduction or the enhancement of cellular properties. It welcomes papers that describe the engineering of native pathways and the synthesis of heterologous pathways to convert microorganisms into microbial cell factories. The journal covers experimental, computational, and modeling approaches for understanding metabolic pathways and manipulating them through genetic, media, or environmental means. Effective exploration of metabolic pathways necessitates the use of molecular biology and biochemistry methods, as well as engineering techniques for modeling and data analysis. MBE serves as a platform for interdisciplinary research in fields such as biochemistry, molecular biology, applied microbiology, cellular physiology, cellular nutrition in health and disease, and biochemical engineering. The journal publishes various types of papers, including original research papers and review papers. It is indexed and abstracted in databases such as Scopus, Embase, EMBiology, Current Contents - Life Sciences and Clinical Medicine, Science Citation Index, PubMed/Medline, CAS and Biotechnology Citation Index.
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