海胆受精后精子特异性非组蛋白染色体蛋白的命运。

M Imschenetzky, M Puchi, R Massone, M Roco, S Gamboa
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摘要

为了确定受精后海胆精子特异性非组蛋白染色体蛋白(Sp NHCP)的转变,通过比较配子和受精卵中分离的NHCP的电泳图谱,研究了其在雄性原核形成期间和之后在受精卵中的存在。采用Western免疫印迹法检测受精卵在授精后不同时间存在的NHCP中Sp NHCP的多克隆抗体。免疫学和电泳研究结果表明,受精后不久,大部分Sp NHCP丢失。从未受精卵中获得的NHCP的电泳模式与从受精卵中分离到的NHCP的电泳模式几乎相同。这种相似性强烈提示未受精卵的NHCP在受精后被保存。
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The fate of sperm specific non-histone chromosomal proteins after fertilization in sea urchins.

To determine the transitions of sea urchin sperm specific non-histone chromosomal proteins (Sp NHCP) following fertilization, their presence in zygotes during and after male pronuclear formation was investigated by comparing the electrophoretic patterns of NHCP obtained from gametes with those isolated from zygotes. Polyclonal antibodies directed against whole Sp NHCP were used to detect, by Western immunoblots, the Sp NHCP among the NHCP present in zygotes at different times post-insemination. The results obtained from immunological and electrophoretic studies indicate that shortly after fertilization the majority of Sp NHCP are lost. The electrophoretic patterns of NHCP obtained from unfertilized eggs were almost identical with those observed for NHCP isolated from zygotes. This similarity strongly suggests that the NHCP of unfertilized eggs are conserved after fertilization.

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