Giulio Camarlinghi , Eva Maria Parisio , Agostino Ognibene
{"title":"I-dOne：临床相关微生物菌株鉴定领域的诊断工具。","authors":"Giulio Camarlinghi , Eva Maria Parisio , Agostino Ognibene","doi":"10.1016/j.mimet.2024.107083","DOIUrl":null,"url":null,"abstract":"<div><div>This study evaluates the performance of I-dOne, the first CE-IVD marked software for microbial species identification based on Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR) and compares its results with MALDI-TOF MS technology (Vitek MS, bioMérieux). A total of 410 clinical isolates were analyzed, spanning 45 species and 24 genera. I-dOne demonstrated a high agreement rate (97.3 %) with the Vitek MS, meeting CLSI standard for microbial identification accuracy. Additionally, this study explored the development of a novel algorithm within I-dOne to discriminate between <em>Bacteroides fragilis</em> and <em>Bacteroides ovatus</em> strains, overcoming the current limitations in species-level differentiation. Finally, the influence of ageing under prolonged aerobic exposure on ATR-FTIR profiles was investigated, highlighting no significant spectral changes in <em>Bacteroides fragilis</em> strains under prolonged aerobic exposure. These findings underscore the accuracy of I-dOne software in microbial identification, offering a reliable alternative to conventional methods.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"228 ","pages":"Article 107083"},"PeriodicalIF":1.7000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"I-dOne: A diagnostic tool in the field of identification of clinically relevant microbial strains\",\"authors\":\"Giulio Camarlinghi , Eva Maria Parisio , Agostino Ognibene\",\"doi\":\"10.1016/j.mimet.2024.107083\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>This study evaluates the performance of I-dOne, the first CE-IVD marked software for microbial species identification based on Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR) and compares its results with MALDI-TOF MS technology (Vitek MS, bioMérieux). A total of 410 clinical isolates were analyzed, spanning 45 species and 24 genera. I-dOne demonstrated a high agreement rate (97.3 %) with the Vitek MS, meeting CLSI standard for microbial identification accuracy. Additionally, this study explored the development of a novel algorithm within I-dOne to discriminate between <em>Bacteroides fragilis</em> and <em>Bacteroides ovatus</em> strains, overcoming the current limitations in species-level differentiation. Finally, the influence of ageing under prolonged aerobic exposure on ATR-FTIR profiles was investigated, highlighting no significant spectral changes in <em>Bacteroides fragilis</em> strains under prolonged aerobic exposure. These findings underscore the accuracy of I-dOne software in microbial identification, offering a reliable alternative to conventional methods.</div></div>\",\"PeriodicalId\":16409,\"journal\":{\"name\":\"Journal of microbiological methods\",\"volume\":\"228 \",\"pages\":\"Article 107083\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of microbiological methods\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0167701224001957\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microbiological methods","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167701224001957","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
I-dOne: A diagnostic tool in the field of identification of clinically relevant microbial strains
This study evaluates the performance of I-dOne, the first CE-IVD marked software for microbial species identification based on Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR) and compares its results with MALDI-TOF MS technology (Vitek MS, bioMérieux). A total of 410 clinical isolates were analyzed, spanning 45 species and 24 genera. I-dOne demonstrated a high agreement rate (97.3 %) with the Vitek MS, meeting CLSI standard for microbial identification accuracy. Additionally, this study explored the development of a novel algorithm within I-dOne to discriminate between Bacteroides fragilis and Bacteroides ovatus strains, overcoming the current limitations in species-level differentiation. Finally, the influence of ageing under prolonged aerobic exposure on ATR-FTIR profiles was investigated, highlighting no significant spectral changes in Bacteroides fragilis strains under prolonged aerobic exposure. These findings underscore the accuracy of I-dOne software in microbial identification, offering a reliable alternative to conventional methods.
期刊介绍：
The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach.
All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
