Yasir Dilshad Siddiqui, Erry Mochamad Arief, Muhammad Aliyan Saddique
{"title":"新型海洋胶原在牙周韧带成纤维细胞上的生物相容性：增强再生治疗的途径。","authors":"Yasir Dilshad Siddiqui, Erry Mochamad Arief, Muhammad Aliyan Saddique","doi":"10.1002/cbf.70040","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Body wall of sea cucumber <i>Bohadschia bivittate</i> contains a protein consisting of highly insoluble collagen fibers. We aimed to evaluate the biocompatibility and cytotoxicity of nonirradiated or <i>γ</i>-irradiated pepsin soluble collagen (PSC) extracted from <i>Bohadschia bivittata</i> on human periodontal ligament fibroblasts cells. The MTT assay showed significant increase in the cell viability values indicating that PSC is noncytotoxic. Further, nonirradiated PSC showed higher cell viabilities values than <i>γ</i>-irradiated PSC at all concentration, especially at an optimal dilution of 25% (<i>p</i> < 0.05). This exploratory study suggests that PSC from <i>Bohadschia bivittata</i> could be utilized as a novel biomaterial in periodontal regenerative therapies.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 1","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Biocompatibility of Novel Marine Collagen on Periodontal Ligament Fibroblasts: A Pathway to Enhanced Regenerative Therapies\",\"authors\":\"Yasir Dilshad Siddiqui, Erry Mochamad Arief, Muhammad Aliyan Saddique\",\"doi\":\"10.1002/cbf.70040\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Body wall of sea cucumber <i>Bohadschia bivittate</i> contains a protein consisting of highly insoluble collagen fibers. We aimed to evaluate the biocompatibility and cytotoxicity of nonirradiated or <i>γ</i>-irradiated pepsin soluble collagen (PSC) extracted from <i>Bohadschia bivittata</i> on human periodontal ligament fibroblasts cells. The MTT assay showed significant increase in the cell viability values indicating that PSC is noncytotoxic. Further, nonirradiated PSC showed higher cell viabilities values than <i>γ</i>-irradiated PSC at all concentration, especially at an optimal dilution of 25% (<i>p</i> < 0.05). This exploratory study suggests that PSC from <i>Bohadschia bivittata</i> could be utilized as a novel biomaterial in periodontal regenerative therapies.</p></div>\",\"PeriodicalId\":9669,\"journal\":{\"name\":\"Cell Biochemistry and Function\",\"volume\":\"43 1\",\"pages\":\"\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cell Biochemistry and Function\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cbf.70040\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Biochemistry and Function","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cbf.70040","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Biocompatibility of Novel Marine Collagen on Periodontal Ligament Fibroblasts: A Pathway to Enhanced Regenerative Therapies
Body wall of sea cucumber Bohadschia bivittate contains a protein consisting of highly insoluble collagen fibers. We aimed to evaluate the biocompatibility and cytotoxicity of nonirradiated or γ-irradiated pepsin soluble collagen (PSC) extracted from Bohadschia bivittata on human periodontal ligament fibroblasts cells. The MTT assay showed significant increase in the cell viability values indicating that PSC is noncytotoxic. Further, nonirradiated PSC showed higher cell viabilities values than γ-irradiated PSC at all concentration, especially at an optimal dilution of 25% (p < 0.05). This exploratory study suggests that PSC from Bohadschia bivittata could be utilized as a novel biomaterial in periodontal regenerative therapies.
期刊介绍：
Cell Biochemistry and Function publishes original research articles and reviews on the mechanisms whereby molecular and biochemical processes control cellular activity with a particular emphasis on the integration of molecular and cell biology, biochemistry and physiology in the regulation of tissue function in health and disease.
The primary remit of the journal is on mammalian biology both in vivo and in vitro but studies of cells in situ are especially encouraged. Observational and pathological studies will be considered providing they include a rational discussion of the possible molecular and biochemical mechanisms behind them and the immediate impact of these observations to our understanding of mammalian biology.
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