猴前额叶皮层神经元兴奋性基因操纵后锥体细胞突触的改变。

IF 2.1 3区医学 Q3 NEUROSCIENCES Journal of neurophysiology Pub Date : 2025-02-01 Epub Date: 2024-12-31 DOI:10.1152/jn.00326.2024

Guillermo Gonzalez-Burgos, Takeaki Miyamae, Yosuke Nishihata, Olga L Krimer, Kirsten Wade, Kenneth N Fish, Dominique Arion, Zhao-Lin Cai, Mingshan Xue, William R Stauffer, David A Lewis

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引用次数: 0

摘要

背外侧前额叶皮层（DLPFC）在灵长类动物的认知中起着至关重要的作用，它整合多模态信息，产生自上而下的认知控制信号。在认知任务中，DLPFC表现出异常复杂和持续时间的活动模式，这在其他皮质区域或物种中是没有观察到的。这些活动模式可能与灵长类DLPFC锥体神经元（PNs）独特的生理和形态特性有关。然而，关于灵长类动物DLPFC的独特体内活性如何调节其PNs的独特特性，人们知之甚少。为了测试在体内操纵猕猴DLPFC 46区的神经元兴奋性是否会影响突触对PNs的输入，我们使用了腺相关病毒载体（AAV）介导的Kir2.1通道的过表达，Kir2.1通道是一种基因沉默工具，先前被证明可以降低体内神经元兴奋性和放电活性。在aav显微注射到DLPFC 46区7至12周后，我们使用膜片钳记录急性切片的PNs来评估Kir2.1过表达的影响。我们发现Kir2.1过表达通过aav编码的Kir2.1通道显著降低PN兴奋性。此外，突触电流记录显示，Kir2.1过表达显著降低兴奋性突触强度，但不影响抑制性突触。因此，我们首次表明，通过aav传递的重组DNA工具改变神经元的兴奋性可以有效地改变灵长类动物新皮层的突触特性。此外，我们报告说，在体内操纵神经元兴奋性影响突触特性的方式在灵长类动物DLPFC网络和啮齿动物皮层之间似乎有所不同。

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Synaptic alterations in pyramidal cells following genetic manipulation of neuronal excitability in monkey prefrontal cortex.

The primate dorsolateral prefrontal cortex (DLPFC) displays unique in vivo activity patterns, but how in vivo activity regulates DLPFC pyramidal neuron (PN) properties remains unclear. We assessed the effects of in vivo Kir2.1 overexpression, a genetic silencing tool, on synapses in monkey DLPFC PNs. We show for the first time that recombinant ion channel expression successfully modifies the excitability of primate cortex neurons, producing effects on synaptic properties apparently different from those in the rodent cortex.

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来源期刊

Journal of neurophysiology 医学-神经科学

CiteScore

4.80

自引率

8.00%

发文量

255

审稿时长

2-3 weeks

期刊介绍： The Journal of Neurophysiology publishes original articles on the function of the nervous system. All levels of function are included, from the membrane and cell to systems and behavior. Experimental approaches include molecular neurobiology, cell culture and slice preparations, membrane physiology, developmental neurobiology, functional neuroanatomy, neurochemistry, neuropharmacology, systems electrophysiology, imaging and mapping techniques, and behavioral analysis. Experimental preparations may be invertebrate or vertebrate species, including humans. Theoretical studies are acceptable if they are tied closely to the interpretation of experimental data and elucidate principles of broad interest.