{"title":"LncRNAs G2E3-AS1和BACE1-AS作为转移性结直肠癌预后生物标志物的上调","authors":"Shahrbanoo Nandoust Kenari, Parisa Mohamadynejad, Mehdi Moghanibashi, Abouzar Bagheri, Leila Rouhi","doi":"10.1080/1354750X.2024.2448508","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Despite the current diagnostic and therapeutic methods for colorectal cancer (CRC), patients are often diagnosed at advanced stages of colorectal cancer. Recently, numerous investigations have highlighted the role of lncRNAs in cancer development and progression. This study investigated less well-characterized genes in the colorectal cancer metastasis process.</p><p><strong>Materials and methods: </strong>Genes expression profiles from CRC patients were downloaded from the TCGA database by the TCGAbiolinks R package. Differential gene expression analysis of miRNA, lncRNAs, and mRNAs was conducted for the M1 and M0 compared to control samples. Then, the DIANA lncbase3 tool was used to find M1-specific miRNA-LncRNA interactions. In addition, the expression of selected genes was evaluated by Real-time RT-PCR in forty-one CRC tissues.</p><p><strong>Results: </strong>Our analysis showed that the expression levels of 77 lncRNAs, 12 miRNAs, and 627 mRNA were significantly changed only in metastatic tumors. In experimental study, significant overexpression of LncRNAs LINC00839, LINC01006, BACE1-AS and G2E3-AS1 was confirmed in metastatic tumors. Also, ROC analysis showed that these lncRNAs, especially lncRNAs G2E3-AS1 and BACE1-AS, are good prognostic biomarkers for metastatic colorectal tumors.</p><p><strong>Conclusion: </strong>We demonstrated that the lncRNAs G2E3-AS1 and BACE1-AS expression upregulated in CRC tissues can be good potential biomarkers for metastatic colorectal cancer.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"1-9"},"PeriodicalIF":2.0000,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Upregulation of LncRNAs G2E3-AS1 and BACE1-AS as prognostic biomarkers in metastatic colorectal cancer.\",\"authors\":\"Shahrbanoo Nandoust Kenari, Parisa Mohamadynejad, Mehdi Moghanibashi, Abouzar Bagheri, Leila Rouhi\",\"doi\":\"10.1080/1354750X.2024.2448508\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Despite the current diagnostic and therapeutic methods for colorectal cancer (CRC), patients are often diagnosed at advanced stages of colorectal cancer. Recently, numerous investigations have highlighted the role of lncRNAs in cancer development and progression. This study investigated less well-characterized genes in the colorectal cancer metastasis process.</p><p><strong>Materials and methods: </strong>Genes expression profiles from CRC patients were downloaded from the TCGA database by the TCGAbiolinks R package. Differential gene expression analysis of miRNA, lncRNAs, and mRNAs was conducted for the M1 and M0 compared to control samples. Then, the DIANA lncbase3 tool was used to find M1-specific miRNA-LncRNA interactions. In addition, the expression of selected genes was evaluated by Real-time RT-PCR in forty-one CRC tissues.</p><p><strong>Results: </strong>Our analysis showed that the expression levels of 77 lncRNAs, 12 miRNAs, and 627 mRNA were significantly changed only in metastatic tumors. In experimental study, significant overexpression of LncRNAs LINC00839, LINC01006, BACE1-AS and G2E3-AS1 was confirmed in metastatic tumors. Also, ROC analysis showed that these lncRNAs, especially lncRNAs G2E3-AS1 and BACE1-AS, are good prognostic biomarkers for metastatic colorectal tumors.</p><p><strong>Conclusion: </strong>We demonstrated that the lncRNAs G2E3-AS1 and BACE1-AS expression upregulated in CRC tissues can be good potential biomarkers for metastatic colorectal cancer.</p>\",\"PeriodicalId\":8921,\"journal\":{\"name\":\"Biomarkers\",\"volume\":\" \",\"pages\":\"1-9\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-01-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomarkers\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/1354750X.2024.2448508\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomarkers","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/1354750X.2024.2448508","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Upregulation of LncRNAs G2E3-AS1 and BACE1-AS as prognostic biomarkers in metastatic colorectal cancer.
Background: Despite the current diagnostic and therapeutic methods for colorectal cancer (CRC), patients are often diagnosed at advanced stages of colorectal cancer. Recently, numerous investigations have highlighted the role of lncRNAs in cancer development and progression. This study investigated less well-characterized genes in the colorectal cancer metastasis process.
Materials and methods: Genes expression profiles from CRC patients were downloaded from the TCGA database by the TCGAbiolinks R package. Differential gene expression analysis of miRNA, lncRNAs, and mRNAs was conducted for the M1 and M0 compared to control samples. Then, the DIANA lncbase3 tool was used to find M1-specific miRNA-LncRNA interactions. In addition, the expression of selected genes was evaluated by Real-time RT-PCR in forty-one CRC tissues.
Results: Our analysis showed that the expression levels of 77 lncRNAs, 12 miRNAs, and 627 mRNA were significantly changed only in metastatic tumors. In experimental study, significant overexpression of LncRNAs LINC00839, LINC01006, BACE1-AS and G2E3-AS1 was confirmed in metastatic tumors. Also, ROC analysis showed that these lncRNAs, especially lncRNAs G2E3-AS1 and BACE1-AS, are good prognostic biomarkers for metastatic colorectal tumors.
Conclusion: We demonstrated that the lncRNAs G2E3-AS1 and BACE1-AS expression upregulated in CRC tissues can be good potential biomarkers for metastatic colorectal cancer.
期刊介绍：
The journal Biomarkers brings together all aspects of the rapidly growing field of biomarker research, encompassing their various uses and applications in one essential source.
Biomarkers provides a vital forum for the exchange of ideas and concepts in all areas of biomarker research. High quality papers in four main areas are accepted and manuscripts describing novel biomarkers and their subsequent validation are especially encouraged:
• Biomarkers of disease
• Biomarkers of exposure
• Biomarkers of response
• Biomarkers of susceptibility
Manuscripts can describe biomarkers measured in humans or other animals in vivo or in vitro. Biomarkers will consider publishing negative data from studies of biomarkers of susceptibility in human populations.
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