Yi Li, Jiaojiao Wang, Chunyuan Wu, Jian He, Qinfen Li, Xiao Deng, Huadong Tan
{"title":"从甜瓜根际土壤中分离出的耐Al3+的耐铝迪氏菌,具有抑制甜瓜尖孢镰刀菌的能力。","authors":"Yi Li, Jiaojiao Wang, Chunyuan Wu, Jian He, Qinfen Li, Xiao Deng, Huadong Tan","doi":"10.1099/ijsem.0.006611","DOIUrl":null,"url":null,"abstract":"<p><p>A bacterial strain, designated as A6<sup>T</sup>, was isolated from the rhizosphere soil of a healthy muskmelon in Wenchang, Hainan Province, China. The cells of strain A6<sup>T</sup> were Gram-negative, aerobic, short rod and motile with a single polar flagellum. Strain A6<sup>T</sup> could tolerate up to 55.0 mM Al<sup>3+</sup> and inhibited the growth of <i>Fusarium oxysporum</i> f. sp. <i>melonis,</i> which is the pathogen of muskmelon <i>Fusarium wilt</i>. Growth occurred at 15-37 ℃ (optimum at 30 ℃), pH 4.5-8.0 (optimum pH 6.5) and with 0-3.0 % NaCl (w/v; optimum, 0.5%). Strain A6<sup>T</sup> shared the highest 16S rRNA gene sequence similarities with <i>Dyella lutea</i> Sa<sup>T</sup> (98.0%), followed by <i>Dyella thiooxydans</i> ATSB10<sup>T</sup> (98.0%), <i>Frateuria edaphi</i> 5GH9-34<sup>T</sup> (97.9%), <i>Dyella nitratireducens</i> DHG59<sup>T</sup> (97.7%), <i>Frateuria defendens</i> DHo<sup>T</sup> (97.7%) and <i>Frateuria soli</i> 5GH9-11<sup>T</sup> (97.7%). Phylogenetic trees based on 16S rRNA gene and genomic sequences indicated that strain A6<sup>T</sup> belonged to the genus <i>Dyella</i> and formed a subclade with <i>Dyella lutea</i> Sa<sup>T</sup> and <i>Dyella thiooxydans</i> ATSB10<sup>T</sup>. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values between A6<sup>T</sup> and its closely related type strains were 78.8-80.8 %, 70.0-71.7 % and 20.5-22.1 %, respectively. The sole respiratory quinone was ubiquinone-8 (Q-8). The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminophospholipids (APL1-2) and three unidentified phospholipids (PL1-3). The major cellular fatty acids (≥5 %) were iso-C<sub>17 : 0</sub>, C<sub>16 : 0</sub>, summed feature 9 (iso-C<sub>17 : 1</sub> <i>ω</i>9<i>c</i> and/or C<sub>16 : 0</sub> 10-methyl), iso-C<sub>15 : 0</sub>, iso-C<sub>16 : 0</sub> and anteiso-C<sub>17 : 0</sub>. The genome size of strain A6<sup>T</sup> was 3.7 Mb with a DNA G+C content of 65.1%. Based on the phenotypic, phylogenetic, genotypic and chemotaxonomic features, strain A6<sup>T</sup> represents a novel species in the genus <i>Dyella</i>, for which <i>Dyella aluminiiresistens</i> A6<sup>T</sup> sp. nov. is proposed. The type strain is A6<sup>T</sup> (= GDMCC 1.4640<sup>T</sup> = KCTC 92542<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Dyella aluminiiresistens</i> sp. nov., a Al<sup>3+</sup>-tolerant bacterium with the ability to inhibit <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> isolated from rhizosphere soil of muskmelon.\",\"authors\":\"Yi Li, Jiaojiao Wang, Chunyuan Wu, Jian He, Qinfen Li, Xiao Deng, Huadong Tan\",\"doi\":\"10.1099/ijsem.0.006611\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A bacterial strain, designated as A6<sup>T</sup>, was isolated from the rhizosphere soil of a healthy muskmelon in Wenchang, Hainan Province, China. The cells of strain A6<sup>T</sup> were Gram-negative, aerobic, short rod and motile with a single polar flagellum. Strain A6<sup>T</sup> could tolerate up to 55.0 mM Al<sup>3+</sup> and inhibited the growth of <i>Fusarium oxysporum</i> f. sp. <i>melonis,</i> which is the pathogen of muskmelon <i>Fusarium wilt</i>. Growth occurred at 15-37 ℃ (optimum at 30 ℃), pH 4.5-8.0 (optimum pH 6.5) and with 0-3.0 % NaCl (w/v; optimum, 0.5%). Strain A6<sup>T</sup> shared the highest 16S rRNA gene sequence similarities with <i>Dyella lutea</i> Sa<sup>T</sup> (98.0%), followed by <i>Dyella thiooxydans</i> ATSB10<sup>T</sup> (98.0%), <i>Frateuria edaphi</i> 5GH9-34<sup>T</sup> (97.9%), <i>Dyella nitratireducens</i> DHG59<sup>T</sup> (97.7%), <i>Frateuria defendens</i> DHo<sup>T</sup> (97.7%) and <i>Frateuria soli</i> 5GH9-11<sup>T</sup> (97.7%). Phylogenetic trees based on 16S rRNA gene and genomic sequences indicated that strain A6<sup>T</sup> belonged to the genus <i>Dyella</i> and formed a subclade with <i>Dyella lutea</i> Sa<sup>T</sup> and <i>Dyella thiooxydans</i> ATSB10<sup>T</sup>. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values between A6<sup>T</sup> and its closely related type strains were 78.8-80.8 %, 70.0-71.7 % and 20.5-22.1 %, respectively. The sole respiratory quinone was ubiquinone-8 (Q-8). The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminophospholipids (APL1-2) and three unidentified phospholipids (PL1-3). The major cellular fatty acids (≥5 %) were iso-C<sub>17 : 0</sub>, C<sub>16 : 0</sub>, summed feature 9 (iso-C<sub>17 : 1</sub> <i>ω</i>9<i>c</i> and/or C<sub>16 : 0</sub> 10-methyl), iso-C<sub>15 : 0</sub>, iso-C<sub>16 : 0</sub> and anteiso-C<sub>17 : 0</sub>. The genome size of strain A6<sup>T</sup> was 3.7 Mb with a DNA G+C content of 65.1%. Based on the phenotypic, phylogenetic, genotypic and chemotaxonomic features, strain A6<sup>T</sup> represents a novel species in the genus <i>Dyella</i>, for which <i>Dyella aluminiiresistens</i> A6<sup>T</sup> sp. nov. is proposed. The type strain is A6<sup>T</sup> (= GDMCC 1.4640<sup>T</sup> = KCTC 92542<sup>T</sup>).</p>\",\"PeriodicalId\":14390,\"journal\":{\"name\":\"International journal of systematic and evolutionary microbiology\",\"volume\":\"75 1\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of systematic and evolutionary microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/ijsem.0.006611\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006611","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Dyella aluminiiresistens sp. nov., a Al3+-tolerant bacterium with the ability to inhibit Fusarium oxysporum f. sp. melonis isolated from rhizosphere soil of muskmelon.
A bacterial strain, designated as A6T, was isolated from the rhizosphere soil of a healthy muskmelon in Wenchang, Hainan Province, China. The cells of strain A6T were Gram-negative, aerobic, short rod and motile with a single polar flagellum. Strain A6T could tolerate up to 55.0 mM Al3+ and inhibited the growth of Fusarium oxysporum f. sp. melonis, which is the pathogen of muskmelon Fusarium wilt. Growth occurred at 15-37 ℃ (optimum at 30 ℃), pH 4.5-8.0 (optimum pH 6.5) and with 0-3.0 % NaCl (w/v; optimum, 0.5%). Strain A6T shared the highest 16S rRNA gene sequence similarities with Dyella lutea SaT (98.0%), followed by Dyella thiooxydans ATSB10T (98.0%), Frateuria edaphi 5GH9-34T (97.9%), Dyella nitratireducens DHG59T (97.7%), Frateuria defendens DHoT (97.7%) and Frateuria soli 5GH9-11T (97.7%). Phylogenetic trees based on 16S rRNA gene and genomic sequences indicated that strain A6T belonged to the genus Dyella and formed a subclade with Dyella lutea SaT and Dyella thiooxydans ATSB10T. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values between A6T and its closely related type strains were 78.8-80.8 %, 70.0-71.7 % and 20.5-22.1 %, respectively. The sole respiratory quinone was ubiquinone-8 (Q-8). The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminophospholipids (APL1-2) and three unidentified phospholipids (PL1-3). The major cellular fatty acids (≥5 %) were iso-C17 : 0, C16 : 0, summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl), iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. The genome size of strain A6T was 3.7 Mb with a DNA G+C content of 65.1%. Based on the phenotypic, phylogenetic, genotypic and chemotaxonomic features, strain A6T represents a novel species in the genus Dyella, for which Dyella aluminiiresistens A6T sp. nov. is proposed. The type strain is A6T (= GDMCC 1.4640T = KCTC 92542T).
期刊介绍:
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.