从白令海和楚科奇海表面海水中分离出来的Tistrella arctic sp. nov.及对Tistrella属的订正描述。

IF 2 3区 生物学 Q4 MICROBIOLOGY International journal of systematic and evolutionary microbiology Pub Date : 2025-01-01 DOI:10.1099/ijsem.0.006627
Jinzhu Zhang, Jianning Wang, Qiliang Lai, Xiupian Liu, Guangyu Li, Panyi Pan, Ziyuan He, Zongze Shao, Chunming Dong
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引用次数: 0

摘要

从白令海和楚科奇海采集的表层海水中分别分离到革兰氏染色阴性、运动、不产孢子、好氧或兼性厌氧、短杆状的2株细菌25B02-3T和BH-R2-4。两株的16S rRNA基因序列相同。对16S rRNA基因序列的系统发育分析表明,该基因序列与Tistrella属同源,与bauzanensis BZ78T和T. mobilis IAM 14872T的序列相似性分别为99.6%和98.2%。基于16S rRNA基因和基因组序列的系统发育分析表明,这两个菌株在Tistrella属内形成了一个单系分支,表明它们可能代表一个新种。两菌株的数字DNA-DNA杂交(dDDH)值和平均核苷酸同一性(ANI)值分别为93和99%,表明它们是不同的菌株。两株与鼠霉属型株的dDDH和ANI值分别为22.4 ~ 58.3%和81.0 ~ 95.0%。这些数据清楚地表明,这两个菌株代表了一个独立的基因组物种的Tistrella属。主要脂肪酸是Sum In Feature 8 (C18: 1 ω7c或C18: 1 ω6c)、C19: 0 cyclo ω8c、Sum In Feature 2 (C12: 0醛或未知10.928)和C16: 0。呼吸醌以Q-10为主,Q-9次之。极性脂质包括磷脂酰乙醇胺、氨基脂和磷脂。菌株25B02-3T和BH-R2-4的基因组DNA G+C含量分别为67.3%和67.4%。根据本研究提供的多相证据,这两个菌株代表了Tistrella属中的一个新种,并建议将其命名为Tistrella arctica sp. 11。型应变为25B02-3T (=MCCC 1A07333T=KCTC 8340T)。
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Tistrella arctica sp. nov., isolated from the surface seawater of the Bering Sea and Chukchi Sea and emended description of the genus Tistrella.

Two Gram-stain-negative, motile, non-spore-forming, aerobic or facultative anaerobic and short rod-shaped bacterial strains, 25B02-3T and BH-R2-4, were isolated from surface seawater collected from the Bering Sea and Chukchi Sea, respectively. The 16S rRNA gene sequences of the two strains were identical. The phylogenetic analysis of the 16S rRNA gene sequences indicated that they were related to the genus Tistrella and shared 99.6 and 98.2% sequence similarity with T. bauzanensis BZ78T and T. mobilis IAM 14872T, respectively. Both 16S rRNA gene and genome sequence-based phylogenetic analyses showed that the two strains formed a monophyletic clade within the genus Tistrella, indicating that they may represent a novel species. The digital DNA‒DNA hybridization (dDDH) values and average nucleotide identities (ANI) between the two strains were 93 and 99%, respectively, indicating that they are different strains. The dDDH and ANI values between the two strains and the type strains of the genus Tistrella were 22.4-58.3% and 81.0-95.0%, respectively. These data clearly demonstrated that the two strains represent a separate genomic species of the genus Tistrella. The principal fatty acids were Sum In Feature 8 (C18 : 1 ω7c or C18 : 1 ω6c), C19 : 0 cyclo ω8c, Sum In Feature 2 (C12 : 0 aldehyde or unknown 10.928) and C16 : 0. The predominant respiratory quinone was Q-10, with a minor Q-9. The polar lipids included phosphatidylethanolamine, aminolipids and phospholipids. The genomic DNA G+C contents of strains 25B02-3T and BH-R2-4 were 67.3 mol% and 67.4 mol%, respectively. On the basis of the polyphasic evidence presented in this study, the two strains represent a novel species within the genus Tistrella, for which the name Tistrella arctica sp. nov. is proposed. The type strain is 25B02-3T (=MCCC 1A07333T=KCTC 8340T).

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来源期刊
CiteScore
5.20
自引率
21.40%
发文量
426
审稿时长
1 months
期刊介绍: Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.
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