{"title":"从白令海和楚科奇海表面海水中分离出来的Tistrella arctic sp. nov.及对Tistrella属的订正描述。","authors":"Jinzhu Zhang, Jianning Wang, Qiliang Lai, Xiupian Liu, Guangyu Li, Panyi Pan, Ziyuan He, Zongze Shao, Chunming Dong","doi":"10.1099/ijsem.0.006627","DOIUrl":null,"url":null,"abstract":"<p><p>Two Gram-stain-negative, motile, non-spore-forming, aerobic or facultative anaerobic and short rod-shaped bacterial strains, 25B02-3<sup>T</sup> and BH-R2-4, were isolated from surface seawater collected from the Bering Sea and Chukchi Sea, respectively. The 16S rRNA gene sequences of the two strains were identical. The phylogenetic analysis of the 16S rRNA gene sequences indicated that they were related to the genus <i>Tistrella</i> and shared 99.6 and 98.2% sequence similarity with <i>T. bauzanensis</i> BZ78<sup>T</sup> and <i>T. mobilis</i> IAM 14872<sup>T</sup>, respectively. Both 16S rRNA gene and genome sequence-based phylogenetic analyses showed that the two strains formed a monophyletic clade within the genus <i>Tistrella</i>, indicating that they may represent a novel species. The digital DNA‒DNA hybridization (dDDH) values and average nucleotide identities (ANI) between the two strains were 93 and 99%, respectively, indicating that they are different strains. The dDDH and ANI values between the two strains and the type strains of the genus <i>Tistrella</i> were 22.4-58.3% and 81.0-95.0%, respectively. These data clearly demonstrated that the two strains represent a separate genomic species of the genus <i>Tistrella</i>. The principal fatty acids were Sum In Feature 8 (C<sub>18 : 1</sub> <i>ω7</i>c or C<sub>18 : 1</sub> <i>ω6</i>c), C<sub>19 : 0</sub> cyclo <i>ω</i>8<i>c</i>, Sum In Feature 2 (C<sub>12 : 0</sub> aldehyde or unknown 10.928) and C<sub>16 : 0</sub>. The predominant respiratory quinone was Q-10, with a minor Q-9. The polar lipids included phosphatidylethanolamine, aminolipids and phospholipids. The genomic DNA G+C contents of strains 25B02-3<sup>T</sup> and BH-R2-4 were 67.3 mol% and 67.4 mol%, respectively. On the basis of the polyphasic evidence presented in this study, the two strains represent a novel species within the genus <i>Tistrella</i>, for which the name <i>Tistrella arctica</i> sp. nov. is proposed. The type strain is 25B02-3<sup>T</sup> (=MCCC 1A07333<sup>T</sup>=KCTC 8340<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Tistrella arctica</i> sp. nov., isolated from the surface seawater of the Bering Sea and Chukchi Sea and emended description of the genus <i>Tistrella</i>.\",\"authors\":\"Jinzhu Zhang, Jianning Wang, Qiliang Lai, Xiupian Liu, Guangyu Li, Panyi Pan, Ziyuan He, Zongze Shao, Chunming Dong\",\"doi\":\"10.1099/ijsem.0.006627\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Two Gram-stain-negative, motile, non-spore-forming, aerobic or facultative anaerobic and short rod-shaped bacterial strains, 25B02-3<sup>T</sup> and BH-R2-4, were isolated from surface seawater collected from the Bering Sea and Chukchi Sea, respectively. The 16S rRNA gene sequences of the two strains were identical. The phylogenetic analysis of the 16S rRNA gene sequences indicated that they were related to the genus <i>Tistrella</i> and shared 99.6 and 98.2% sequence similarity with <i>T. bauzanensis</i> BZ78<sup>T</sup> and <i>T. mobilis</i> IAM 14872<sup>T</sup>, respectively. Both 16S rRNA gene and genome sequence-based phylogenetic analyses showed that the two strains formed a monophyletic clade within the genus <i>Tistrella</i>, indicating that they may represent a novel species. The digital DNA‒DNA hybridization (dDDH) values and average nucleotide identities (ANI) between the two strains were 93 and 99%, respectively, indicating that they are different strains. The dDDH and ANI values between the two strains and the type strains of the genus <i>Tistrella</i> were 22.4-58.3% and 81.0-95.0%, respectively. These data clearly demonstrated that the two strains represent a separate genomic species of the genus <i>Tistrella</i>. The principal fatty acids were Sum In Feature 8 (C<sub>18 : 1</sub> <i>ω7</i>c or C<sub>18 : 1</sub> <i>ω6</i>c), C<sub>19 : 0</sub> cyclo <i>ω</i>8<i>c</i>, Sum In Feature 2 (C<sub>12 : 0</sub> aldehyde or unknown 10.928) and C<sub>16 : 0</sub>. The predominant respiratory quinone was Q-10, with a minor Q-9. The polar lipids included phosphatidylethanolamine, aminolipids and phospholipids. The genomic DNA G+C contents of strains 25B02-3<sup>T</sup> and BH-R2-4 were 67.3 mol% and 67.4 mol%, respectively. On the basis of the polyphasic evidence presented in this study, the two strains represent a novel species within the genus <i>Tistrella</i>, for which the name <i>Tistrella arctica</i> sp. nov. is proposed. The type strain is 25B02-3<sup>T</sup> (=MCCC 1A07333<sup>T</sup>=KCTC 8340<sup>T</sup>).</p>\",\"PeriodicalId\":14390,\"journal\":{\"name\":\"International journal of systematic and evolutionary microbiology\",\"volume\":\"75 1\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of systematic and evolutionary microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/ijsem.0.006627\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006627","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Tistrella arctica sp. nov., isolated from the surface seawater of the Bering Sea and Chukchi Sea and emended description of the genus Tistrella.
Two Gram-stain-negative, motile, non-spore-forming, aerobic or facultative anaerobic and short rod-shaped bacterial strains, 25B02-3T and BH-R2-4, were isolated from surface seawater collected from the Bering Sea and Chukchi Sea, respectively. The 16S rRNA gene sequences of the two strains were identical. The phylogenetic analysis of the 16S rRNA gene sequences indicated that they were related to the genus Tistrella and shared 99.6 and 98.2% sequence similarity with T. bauzanensis BZ78T and T. mobilis IAM 14872T, respectively. Both 16S rRNA gene and genome sequence-based phylogenetic analyses showed that the two strains formed a monophyletic clade within the genus Tistrella, indicating that they may represent a novel species. The digital DNA‒DNA hybridization (dDDH) values and average nucleotide identities (ANI) between the two strains were 93 and 99%, respectively, indicating that they are different strains. The dDDH and ANI values between the two strains and the type strains of the genus Tistrella were 22.4-58.3% and 81.0-95.0%, respectively. These data clearly demonstrated that the two strains represent a separate genomic species of the genus Tistrella. The principal fatty acids were Sum In Feature 8 (C18 : 1ω7c or C18 : 1ω6c), C19 : 0 cyclo ω8c, Sum In Feature 2 (C12 : 0 aldehyde or unknown 10.928) and C16 : 0. The predominant respiratory quinone was Q-10, with a minor Q-9. The polar lipids included phosphatidylethanolamine, aminolipids and phospholipids. The genomic DNA G+C contents of strains 25B02-3T and BH-R2-4 were 67.3 mol% and 67.4 mol%, respectively. On the basis of the polyphasic evidence presented in this study, the two strains represent a novel species within the genus Tistrella, for which the name Tistrella arctica sp. nov. is proposed. The type strain is 25B02-3T (=MCCC 1A07333T=KCTC 8340T).
期刊介绍:
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.