Likang Wu, Rongfang Chen, Wenxiao Jin, Canwei Peng, Li Wang, Longfei Miao, Yonghai Song
{"title":"基于电活性COFs作为信号探针的碳水化合物抗原CA125电化学免疫传感器。","authors":"Likang Wu, Rongfang Chen, Wenxiao Jin, Canwei Peng, Li Wang, Longfei Miao, Yonghai Song","doi":"10.1016/j.talanta.2025.127546","DOIUrl":null,"url":null,"abstract":"<div><div>Levels of CA125 are strongly associated with cervical, pancreatic, bowel and breast cancer. However, the common CA125 detection method has the disadvantages of poor repeatability, high cost, easy to be disturbed and poor stability. In this work, a COF based electrochemical immunosensor was developed for the rapid, sensitive and stable detection of CA125. The COF<sub>Dha-Tab</sub> synthesized by 2,5-dihydroxyp-phenyldiformaldehyde (Dha) and 1,3,5-tris(4-aminophenyl)benzene (Tab) can better absorb primary antibody (Ab<sub>1</sub>) of CA125 after epoxidation, and the COF<sub>DAAQ-TFP</sub> synthesized by 2,6-diaminoanthraquinone (DAAQ) and 2,4,6-triformylphloroglucinol (TFP) was modified with AuNPs, which can not only improve the electrochemical performance but also provide a platform for loading secondary antibody (Ab<sub>2</sub>). CA125 can be specifically recognized and captured by EP-COF<sub>Dha-Tab</sub> adsorbed to Ab<sub>1</sub>, which can further bind the signal probe AuNPs@COF<sub>DAAQ-TFP</sub>/Ab<sub>2</sub>. The signal response of the electroactive molecule COF<sub>DAAQ-TFP</sub> is positively correlated with the concentration of CA125 to achieve the quantitative detection of CA125.The sensor exhibited a low detection limit of 0.0067 U/mL and a linear range from 0.01 to 100 U/mL. This work presents a novel approach utilizing electroactive COFs as signal probes for the sensitive and quantitative detection of carbohydrate antigens.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"Article 127546"},"PeriodicalIF":6.1000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An electrochemical immunosensor for carbohydrate antigen CA125 based on electroactive COFs as a signal probe\",\"authors\":\"Likang Wu, Rongfang Chen, Wenxiao Jin, Canwei Peng, Li Wang, Longfei Miao, Yonghai Song\",\"doi\":\"10.1016/j.talanta.2025.127546\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Levels of CA125 are strongly associated with cervical, pancreatic, bowel and breast cancer. However, the common CA125 detection method has the disadvantages of poor repeatability, high cost, easy to be disturbed and poor stability. In this work, a COF based electrochemical immunosensor was developed for the rapid, sensitive and stable detection of CA125. The COF<sub>Dha-Tab</sub> synthesized by 2,5-dihydroxyp-phenyldiformaldehyde (Dha) and 1,3,5-tris(4-aminophenyl)benzene (Tab) can better absorb primary antibody (Ab<sub>1</sub>) of CA125 after epoxidation, and the COF<sub>DAAQ-TFP</sub> synthesized by 2,6-diaminoanthraquinone (DAAQ) and 2,4,6-triformylphloroglucinol (TFP) was modified with AuNPs, which can not only improve the electrochemical performance but also provide a platform for loading secondary antibody (Ab<sub>2</sub>). CA125 can be specifically recognized and captured by EP-COF<sub>Dha-Tab</sub> adsorbed to Ab<sub>1</sub>, which can further bind the signal probe AuNPs@COF<sub>DAAQ-TFP</sub>/Ab<sub>2</sub>. The signal response of the electroactive molecule COF<sub>DAAQ-TFP</sub> is positively correlated with the concentration of CA125 to achieve the quantitative detection of CA125.The sensor exhibited a low detection limit of 0.0067 U/mL and a linear range from 0.01 to 100 U/mL. This work presents a novel approach utilizing electroactive COFs as signal probes for the sensitive and quantitative detection of carbohydrate antigens.</div></div>\",\"PeriodicalId\":435,\"journal\":{\"name\":\"Talanta\",\"volume\":\"286 \",\"pages\":\"Article 127546\"},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Talanta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0039914025000323\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0039914025000323","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/7 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
An electrochemical immunosensor for carbohydrate antigen CA125 based on electroactive COFs as a signal probe
Levels of CA125 are strongly associated with cervical, pancreatic, bowel and breast cancer. However, the common CA125 detection method has the disadvantages of poor repeatability, high cost, easy to be disturbed and poor stability. In this work, a COF based electrochemical immunosensor was developed for the rapid, sensitive and stable detection of CA125. The COFDha-Tab synthesized by 2,5-dihydroxyp-phenyldiformaldehyde (Dha) and 1,3,5-tris(4-aminophenyl)benzene (Tab) can better absorb primary antibody (Ab1) of CA125 after epoxidation, and the COFDAAQ-TFP synthesized by 2,6-diaminoanthraquinone (DAAQ) and 2,4,6-triformylphloroglucinol (TFP) was modified with AuNPs, which can not only improve the electrochemical performance but also provide a platform for loading secondary antibody (Ab2). CA125 can be specifically recognized and captured by EP-COFDha-Tab adsorbed to Ab1, which can further bind the signal probe AuNPs@COFDAAQ-TFP/Ab2. The signal response of the electroactive molecule COFDAAQ-TFP is positively correlated with the concentration of CA125 to achieve the quantitative detection of CA125.The sensor exhibited a low detection limit of 0.0067 U/mL and a linear range from 0.01 to 100 U/mL. This work presents a novel approach utilizing electroactive COFs as signal probes for the sensitive and quantitative detection of carbohydrate antigens.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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