乙肝表面抗原和抗乙肝双阳性慢性乙型肝炎患者的预后和免疫库特征。

IF 3.2 4区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Clinical and Experimental Medicine Pub Date : 2025-01-08 DOI:10.1007/s10238-024-01537-3
Huijun Liang, Haifang Wang, Minfeng Liang, Xiaobin Zhang, Meifen Dai, Haixia Li, Xin Li, Xiaofeng Yin, Xinyao Liu, Jiaqi Yao, Ziyun Guan, Yurong Qiu
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引用次数: 0

摘要

在一些慢性乙型肝炎(CHB)患者(DP患者)中观察到乙型肝炎表面抗原(HBsAg)和乙型肝炎表面抗体(anti-HBs)共存,但这一特定人群的临床结局和免疫微环境变化的综合表征仍不确定。本研究回顾性分析广东省佛山市305例CHB患者的预后,并采用T细胞受体(TCR)和B细胞受体(BCR)免疫库测序方法,研究HBsAg和抗- hbs双阳性CHB患者(DP组)、IFN- γ治疗后恢复的CHB患者(RP组)和健康对照(HC组)的分子免疫学变化。我们的研究结果显示,22.30%的DP患者被诊断为严重的肝脏疾病。免疫库测序显示,与RP组相比,DP组T细胞受体β链(TRB)和免疫球蛋白重链(IGH)的多样性明显偏斜。独特的V(D)J基因组合,如IGHV1-18/IGHD3-22/IGHJ5、IGHV1-8/IGHD6-13/IGHJ3、IGHV1-8/IGHD6-19/IGHJ3,以及TRBV12-3/TRBD1/TRBJ1-5和TRBV11-2/TRBD2/TRBJ2-1,在DP组中表现出不同的利用模式。此外,DP组TRB互补决定区3 (CDR3)中利用最多的前10个氨基酸基序与RP组差异显著。值得注意的是,IGH CDR3s中的“xxxYDSSGYx”和“AREx”等基序在DP组中有选择性地普遍存在。这些发现有望为DP患者临床预后不良提供证据,并为该群体独特的免疫微环境变化提供新的见解。
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The prognosis and immune repertoire characteristics of HBsAg and anti-HBs double positive chronic hepatitis B patients.

Coexistence of hepatitis B surface antigen (HBsAg) and hepatitis B surface antibody (anti-HBs) has been observed in some chronic hepatitis B (CHB) patients (DP patients), but the clinical outcomes and comprehensive characterization of immune micro-environmental changes for this specific population remain inconclusive. In this study, we retrospectively analyze the prognosis of 305 patients in Foshan City, Guangdong Province, China, and also investigated the molecular immunology changes in HBsAg and anti-HBs double positive CHB patients (DP group), CHB patients who had recovered from IFN-ɑ treatment (RP group), and healthy controls (HC group) using T cell receptor (TCR) and B cell receptor (BCR) immune repertoire sequencing. Our findings revealed that 22.30% of DP patients were diagnosed with severe liver disease. Immune repertoire sequencing revealed significant skewing in the diversities of T cell receptor β-chain (TRB) and immunoglobulin heavy chain (IGH) in the DP group compared to the RP group. Unique V(D)J gene combinations, such as IGHV1-18/IGHD3-22/IGHJ5, IGHV1-8/IGHD6-13/IGHJ3, and IGHV1-8/IGHD6-19/IGHJ3, as well as TRBV12-3/TRBD1/TRBJ1-5 and TRBV11-2/TRBD2/TRBJ2-1, exhibited distinct utilization patterns in the DP group. Moreover, the top ten most utilized amino acid motifs in the complementarity determining region 3 (CDR3) of TRB in the DP group showed significant differences from those in the RP group. Notably, motifs such as "xxxYDSSGYx" and "AREx" in the IGH CDR3s were selectively prevalent in the DP group. These findings are expected to provide evidence supporting the poor clinical prognosis of DP patients and offer new insights into the distinct immune micro-environmental changes of this group.

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来源期刊
Clinical and Experimental Medicine
Clinical and Experimental Medicine 医学-医学:研究与实验
CiteScore
4.80
自引率
2.20%
发文量
159
审稿时长
2.5 months
期刊介绍: Clinical and Experimental Medicine (CEM) is a multidisciplinary journal that aims to be a forum of scientific excellence and information exchange in relation to the basic and clinical features of the following fields: hematology, onco-hematology, oncology, virology, immunology, and rheumatology. The journal publishes reviews and editorials, experimental and preclinical studies, translational research, prospectively designed clinical trials, and epidemiological studies. Papers containing new clinical or experimental data that are likely to contribute to changes in clinical practice or the way in which a disease is thought about will be given priority due to their immediate importance. Case reports will be accepted on an exceptional basis only, and their submission is discouraged. The major criteria for publication are clarity, scientific soundness, and advances in knowledge. In compliance with the overwhelmingly prevailing request by the international scientific community, and with respect for eco-compatibility issues, CEM is now published exclusively online.
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