{"title":"银屑病间充质干细胞衍生外泌体的蛋白质组学分析。","authors":"Siyu Wang, Qixin Han, Jun Wang, Xinxin Peng, Ling Zhou, Xuping Niu","doi":"10.2174/0115665240304551241017050628","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to investigate the unique proteins in exosomes from mesenchymal stem cells derived from psoriatic lesions and compare them with those from healthy human skin. It seeks to identify potential regulatory factors that may influence the differential effects observed in these exosomes.</p><p><strong>Methods: </strong>Dermal mesenchymal stem cell exosomes were isolated from healthy human skin (HDMSCs-EXO) and psoriatic lesion of patient (PDMSCs-EXO). The extracted exosomes were analyzed through label-free quantitative proteomics to identify differential proteins. Bioinformatics analyses, including GO and KEGG enrichment, were conducted.</p><p><strong>Results: </strong>The comparative analysis using HDMSCs-EXO as a control group revealed 13 differential proteins. Notably, proteins such as B2R4D5, MFGE8, and MFAP5 are associated with the inflammatory mechanisms of psoriasis.</p><p><strong>Conclusion: </strong>The study identifies several differentially expressed proteins in exosomes may play roles in the development of psoriasis. These finding offer valuable insights for further exploration of the inflammatory processes in psoriasis.</p>","PeriodicalId":10873,"journal":{"name":"Current molecular medicine","volume":" ","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Proteomic Analysis of Mesenchymal Stem Cell-derived Exosomes in Psoriasis.\",\"authors\":\"Siyu Wang, Qixin Han, Jun Wang, Xinxin Peng, Ling Zhou, Xuping Niu\",\"doi\":\"10.2174/0115665240304551241017050628\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>This study aims to investigate the unique proteins in exosomes from mesenchymal stem cells derived from psoriatic lesions and compare them with those from healthy human skin. It seeks to identify potential regulatory factors that may influence the differential effects observed in these exosomes.</p><p><strong>Methods: </strong>Dermal mesenchymal stem cell exosomes were isolated from healthy human skin (HDMSCs-EXO) and psoriatic lesion of patient (PDMSCs-EXO). The extracted exosomes were analyzed through label-free quantitative proteomics to identify differential proteins. Bioinformatics analyses, including GO and KEGG enrichment, were conducted.</p><p><strong>Results: </strong>The comparative analysis using HDMSCs-EXO as a control group revealed 13 differential proteins. Notably, proteins such as B2R4D5, MFGE8, and MFAP5 are associated with the inflammatory mechanisms of psoriasis.</p><p><strong>Conclusion: </strong>The study identifies several differentially expressed proteins in exosomes may play roles in the development of psoriasis. These finding offer valuable insights for further exploration of the inflammatory processes in psoriasis.</p>\",\"PeriodicalId\":10873,\"journal\":{\"name\":\"Current molecular medicine\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-01-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current molecular medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2174/0115665240304551241017050628\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current molecular medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2174/0115665240304551241017050628","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
Proteomic Analysis of Mesenchymal Stem Cell-derived Exosomes in Psoriasis.
Purpose: This study aims to investigate the unique proteins in exosomes from mesenchymal stem cells derived from psoriatic lesions and compare them with those from healthy human skin. It seeks to identify potential regulatory factors that may influence the differential effects observed in these exosomes.
Methods: Dermal mesenchymal stem cell exosomes were isolated from healthy human skin (HDMSCs-EXO) and psoriatic lesion of patient (PDMSCs-EXO). The extracted exosomes were analyzed through label-free quantitative proteomics to identify differential proteins. Bioinformatics analyses, including GO and KEGG enrichment, were conducted.
Results: The comparative analysis using HDMSCs-EXO as a control group revealed 13 differential proteins. Notably, proteins such as B2R4D5, MFGE8, and MFAP5 are associated with the inflammatory mechanisms of psoriasis.
Conclusion: The study identifies several differentially expressed proteins in exosomes may play roles in the development of psoriasis. These finding offer valuable insights for further exploration of the inflammatory processes in psoriasis.
期刊介绍：
Current Molecular Medicine is an interdisciplinary journal focused on providing the readership with current and comprehensive reviews/ mini-reviews, original research articles, short communications/letters and drug clinical trial studies on fundamental molecular mechanisms of disease pathogenesis, the development of molecular-diagnosis and/or novel approaches to rational treatment. The reviews should be of significant interest to basic researchers and clinical investigators in molecular medicine. Periodically the journal invites guest editors to devote an issue on a basic research area that shows promise to advance our understanding of the molecular mechanism(s) of a disease or has potential for clinical applications.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
