结合体内双光子和激光散斑显微镜与离体毛细血管-实质小动脉制备作为研究神经血管耦合的新方法。

IF 1.9 4区 医学 Q3 HEMATOLOGY Microcirculation Pub Date : 2025-01-07 DOI:10.1111/micc.70001
Lowri E. Evans, Anna L. Gray, Katy R. Walsh, Thea G. E. Danby, Harry A. T. Pritchard, Stuart M. Allan, Alison M. Gurney, Adam S. Greenstein, Ingo Schiessl
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引用次数: 0

摘要

目的:脑血流量(CBF)下降越来越被认为是针对神经退行性疾病的一个重要领域,但对CBF变化背后的机制还缺乏充分的了解。动物模型对于扩大我们的知识至关重要,因为研究人类整体脑血流和神经血管耦合的方法有限,并且需要昂贵的专业扫描仪。方法:采用合适的动物模型可以增加我们对脑血管功能的认识,因此我们将慢性颅窗与体内双光子和激光散斑显微镜以及离体毛细血管实质小动脉(CaPA)制备相结合。慢性颅窗允许纵向直接观察大脑微血管和周围实质,而CaPA制备可以在分离的神经元组织中评估毛细血管和小动脉的功能。结果:我们发现硬膜外颅窗和相关成像方案不影响CaPA制备中的血管功能。因此,通过对动物皮质血管进行成像,可以发现导致任何观察到的体内变化的脑血管系统的生理改变。结论:该方法将加强神经退行性疾病的研究,并有利于限制动物的使用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Combining In Vivo Two-Photon and Laser Speckle Microscopy With the Ex Vivo Capillary-Parenchymal Arteriole Preparation as a Novel Approach to Study Neurovascular Coupling

Objective

Cerebral blood flow (CBF) decline is increasingly recognized as an area of importance for targeting neurodegenerative disorders, yet full understanding of the mechanisms that underlie CBF changes are lacking. Animal models are crucial for expanding our knowledge as methods for studying global CBF and neurovascular coupling in humans are limited and require expensive specialized scanners.

Methods

Use of appropriate animal models can increase our understanding of cerebrovascular function, so we have combined chronic cranial windows with in vivo two-photon and laser speckle microscopy and ex vivo capillary-parenchymal arteriole (CaPA) preparations. Chronic cranial windows allow for longitudinal direct observation of the cerebral microvasculature and surrounding parenchyma while the CaPA preparation can assess capillary and arteriole function in isolation of the neuronal tissue.

Results

Here, we found that extra-dural cranial windows and related imaging protocols do not affect vascular function in the CaPA preparation. Cortical vessels from animals that have undergone imaging can therefore be taken to discover physiological alterations in the cerebral vasculature that contribute to any observed in vivo changes.

Conclusion

This approach will enhance neurodegenerative research with the benefit of limiting animal usage.

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来源期刊
Microcirculation
Microcirculation 医学-外周血管病
CiteScore
5.00
自引率
4.20%
发文量
43
审稿时长
6-12 weeks
期刊介绍: The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.
期刊最新文献
Small Arteries From Old Spontaneously Hypertensive Rats Exhibit Enhanced Endothelium-Independent Vasodilatory Capacity and Reduced Stiffness Simulation of Conducted Responses in Microvascular Networks: Role of Gap Junction Current Rectification Different Transcriptome Signatures of the Lymphatic and the Blood Vessels From Rat Mesentery Reveal Distinct Function Characteristics Issue Information Combining In Vivo Two-Photon and Laser Speckle Microscopy With the Ex Vivo Capillary-Parenchymal Arteriole Preparation as a Novel Approach to Study Neurovascular Coupling
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