阿奇霉素负载PLGA微球包被丝素改善炎症和促进牙周组织再生。

IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Regenerative Biomaterials Pub Date : 2024-12-14 eCollection Date: 2025-01-01 DOI:10.1093/rb/rbae146
Zhaoguang Ouyang, Xiaoyu Chen, Zhengyang Wang, Yue Xu, Zhe Deng, Liangyu Xing, Li Zhang, Meilin Hu, Haocong Li, Tengye Lian, Feng Gao, Chunyi Liu, Yangyang Zhou, Lu Sun, Ying ChengYao Wang, Dayong Liu
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引用次数: 0

摘要

牙周炎是一种广泛存在的炎症性疾病,是成年人牙齿脱落的主要原因。机械牙周治疗有利于牙周病的治疗,但辅助牙周治疗也是必要的。局部应用抗炎药在牙周炎治疗中引起了相当大的关注。虽然阿奇霉素(AZM)具有优异的抗炎特性,但由于水溶性差和缺乏缓释机制,其生物利用度受到限制。在此,我们合成了可生物降解的微球(AZM@PLGA-SF),用于持续释放AZM,以局部改善牙周炎症并促进牙周组织再生。采用单乳液-溶剂蒸发法将AZM包封在聚乳酸-羟基乙酸(PLGA)微球(AZM@PLGA)中,然后通过静电吸附在表面涂上丝素(SF),减少AZM的初始爆发释放。在体内,AZM@PLGA-SF微球局部治疗可显著减少牙周炎症,使牙周组织恢复到健康水平。机械上,配方微球通过降低龈沟液中促炎细胞因子(肿瘤坏死因子-α、白细胞介素[IL]-6、干扰素-γ、IL-2和IL- 17a)的水平,促进抗炎细胞因子(IL-4和IL-10)的表达,调节牙周炎症微环境。AZM@PLGA-SF微球具有良好的生物安全性。因此,我们介绍了一种治疗牙周炎的抗炎疗法,它具有减轻牙周炎症和促进牙周组织修复和再生的巨大潜力。
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Azithromycin-loaded PLGA microspheres coated with silk fibroin ameliorate inflammation and promote periodontal tissue regeneration.

Periodontitis, a widespread inflammatory disease, is the major cause of tooth loss in adults. While mechanical periodontal therapy benefits the periodontal disease treatment, adjunctive periodontal therapy is also necessary. Topically applied anti-inflammatory agents have gained considerable attention in periodontitis therapy. Although azithromycin (AZM) possesses excellent anti-inflammatory properties, its bioavailability is limited owing to poor water solubility and the absence of sustained release mechanisms. Herein, we synthesized biodegradable microspheres (AZM@PLGA-SF) for sustained AZM release to locally ameliorate periodontal inflammation and facilitate periodontal tissue regeneration. AZM was encapsulated in poly (lactic-co-glycolic acid) (PLGA) microspheres (AZM@PLGA) using single emulsion-solvent evaporation, followed by surface coating with silk fibroin (SF) via electrostatic adsorption, reducing the initial burst release of AZM. In vivo, local treatment with AZM@PLGA-SF microspheres significantly reduced periodontal inflammation and restored periodontal tissue to healthy levels. Mechanically, the formulated microspheres regulated the periodontal inflammatory microenvironment by reducing the levels of pro-inflammatory cytokines (tumor necrosis factor -α, interleukin [IL]-6, interferon-γ, IL-2, and IL-17A) in gingival crevicular fluid and promoted the expression of anti-inflammatory cytokines (IL-4 and IL-10). AZM@PLGA-SF microspheres demonstrated excellent biological safety. Therefore, we introduce an anti-inflammatory therapy for periodontitis with substantial potential for mitigating periodontal inflammation and encouraging the repair and regeneration of periodontal tissues.

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来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
期刊最新文献
pH-responsive hydrogel with dual-crosslinked network of polyvinyl alcohol/boric acid for controlled release of salvianolic acid B: novel pro-regenerative mechanisms in scar inhibition and wound healing. CO-loaded hemoglobin/EGCG nanoparticles functional coatings for inflammation modulation of vascular implants. Semi-quantitative scoring criteria based on multiple staining methods combined with machine learning to evaluate residual nuclei in decellularized matrix. Injectable microspheres filled with copper-containing bioactive glass improve articular cartilage healing by regulating inflammation and recruiting stem cells. Azithromycin-loaded PLGA microspheres coated with silk fibroin ameliorate inflammation and promote periodontal tissue regeneration.
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