Pei-Yu Han , Zhen-Ni Yin , Jing-Jing Yang , Meng-Si Zhang , Ying Huang , Jian Zhang , Quan-You Lu
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引用次数: 0
摘要
桑树皱叶病毒（Mulberry crcrickleaf virus， MCLV）是双病毒科mulcrcrilvirus属的代表种。在这里，我们发现了一个额外的V6 ORF，它嵌入在MCLV病毒粒子传感链的V4 ORF中。通过分析V6 ORF上游序列的启动子活性和对V6突变体mclv感染番茄植株的病毒DNA积累量进行定量分析，证实了V6的表达。构建了V6突变体MCLV vII （pCA-1.1MCLVmv6）和V4、V6双突变体MCLV vII （pCA-1.1MCLVmV4mV6）的侵染克隆，并利用构建体转染本烟原生质体侵染番茄进行了实验。结果表明，V6在原生质体中MCLV的增殖中起着关键作用，尽管它对MCLV在植物体内的全身感染的影响很小。此外，V4和V6以协同形式正向调节MCLV DNA复制。
V6 encoded by mulberry crinkle leaf virus is important for viral DNA replication
Mulberry crinkle leaf virus (MCLV) is a representative species of the genus Mulcrilevirus in the family Geminiviridae. Here, we identified an additional V6 ORF which embedded within the V4 ORF in the MCLV virion-sense strand. The expression of V6 was confirmed by analyzing the promoter activity of V6 ORF upstream sequences and quantifying the viral DNA accumulation in V6-mutant MCLV-infected tomato plants. Infectious clones of V6-mutant MCLV vII (pCA-1.1MCLVmv6) and V4 and V6 double-mutant MCLV vII (pCA-1.1MCLVmV4mV6) were constructed, and experiments transfecting protoplasts isolated from Nicotiana benthamiana and infecting tomato using the constructs were performed. The results indicate that V6 plays a critical role in the multiplication of MCLV in protoplasts, although it has minimal, if any, impact on the systemic infection of MCLV in plants. Additionally, V4 and V6 positively regulate the MCLV DNA replication in synergistic form.
期刊介绍：
Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.
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