维生素E可以改善接受布硫凡化疗的雄性大鼠的生殖系统。

IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Cellular and molecular biology Pub Date : 2025-01-12 DOI:10.14715/cmb/2024.70.12.24
Ameer Hameed Kadhim, Mehdi El Arbi, Hayder Ali Muhammed
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引用次数: 0

摘要

维生素E是一种众所周知的抗氧化剂,经常被用作癌症治疗的辅助治疗。布磺胺是一种常用的癌症治疗药物。在这项研究中,28只10周龄、体重在250到300克之间的雄性大鼠被分成四组。第一组为对照组,连续4周每天腹腔注射二甲亚砜(DMSO)。第二组腹腔注射单剂量40 mg/kg体重的布硫凡。第三组给予相同单剂量的布苏凡,同时每天腹腔注射维生素E,剂量为100 mg/kg体重,连续四周。第四组连续四周只服用相同剂量的维生素E。实验结束后,处死动物,采集血液,检测抗氧化酶(GSH、SOD、CAT)水平,分析血清生殖激素(FSH、LH、睾酮)浓度。此外,收集附睾精子后评估精子活力和活力。结果显示,Busulfan显著提高血清FSH和LH水平,同时显著降低睾酮水平。此外,Busulfan治疗导致精子数量、活力和活力显著减少,同时精子形态异常显著增加。相比之下,补充维生素E和Busulfan可以提高激素水平,增强精子功能。综上所述,Busulfan对精子有毒性作用,并直接影响体重和睾丸重量。然而,维生素E显示有益的治疗作用,对睾丸组织和提高精子生产的大鼠治疗布苏凡。
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Vitamin E improves the reproductive system of male rats exposed to busulfan chemotherapy.

Vitamin E is a well-known antioxidant and is frequently used as an adjunct treatment in cancer therapy. Busulfan is a commonly used drug for cancer treatment. In this study, twenty-eight male rats, ten weeks old and weighing between 250 and 300 grams, were divided into four groups. The first group served as the control and received daily intraperitoneal injections of Dimethyl sulfoxide (DMSO) for four weeks. The second group received a single dose of Busulfan at 40 mg/kg body weight via intraperitoneal injection. The third group received the same single dose of Busulfan along with daily intraperitoneal injections of Vitamin E at 100 mg/kg body weight for four weeks. The fourth group was given only Vitamin E at the same dosage for four weeks. At the end of the experiment, the animals were sacrificed, and blood samples were collected to test antioxidant enzyme levels (GSH, SOD, CAT) and analyze serum concentrations of reproductive hormones (FSH, LH, and testosterone). Additionally, sperm motility and viability were assessed after collecting epididymal spermatozoa. The findings revealed that Busulfan significantly increased serum levels of both FSH and LH while causing a notable decrease in testosterone levels. Furthermore, Busulfan treatment resulted in a significant reduction in sperm count, motility, and viability, along with a marked increase in sperm morphological abnormalities. In contrast, supplementation with Vitamin E alongside Busulfan improved hormone levels and enhanced sperm function. In conclusion, Busulfan has a toxic effect on sperm and directly impacts body weight and testicular weight. However, Vitamin E demonstrates beneficial therapeutic effects on testicular tissue and enhances sperm production in rats treated with Busulfan.

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来源期刊
Cellular and molecular biology
Cellular and molecular biology 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
331
期刊介绍: Cellular and Molecular Biology publishes original articles, reviews, short communications, methods, meta-analysis notes, letters to editor and comments in the interdisciplinary science of Cellular and Molecular Biology linking and integrating molecular biology, biophysics, biochemistry, enzymology, physiology and biotechnology in a dynamic cell and tissue biology environment, applied to human, animals, plants tissues as well to microbial and viral cells. The journal Cellular and Molecular Biology is therefore open to intense interdisciplinary exchanges in medical, dental, veterinary, pharmacological, botanical and biological researches for the demonstration of these multiple links.
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