多发性骨髓瘤、髓外疾病和浆细胞白血病患者骨髓浆细胞外囊泡的MicroRNA谱分析

IF 3.3 4区 医学 Q2 HEMATOLOGY Hematological Oncology Pub Date : 2025-01-13 DOI:10.1002/hon.70036
Jana Gregorova, Monika Vlachova, Petra Vychytilova-Faltejskova, Adela Dostalova, Tereza Ruzickova, Marek Vecera, Lenka Radova, Vendula Pospichalova, Stanislava Sladecek, Martina Hyzdalova, Jana Kotaskova, Marie Jarosova, Josef Masek, Klara Benesova, Jiri Jarkovsky, Lucie Rihova, Renata Bezdekova, Martina Almasi, Ivanna Boichuk, Martin Stork, Ludek Pour, Sabina Sevcikova
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引用次数: 0

摘要

多发性骨髓瘤是一种浆细胞恶性肿瘤,其特点是单克隆免疫球蛋白异常增多。尽管治疗取得了重大进展,但一些患者仍会发展为更具侵袭性的多发性骨髓瘤,包括髓外疾病或浆细胞白血病。虽然确切的分子机制尚不清楚,但一些研究已经证实,富含细胞外小囊泡的微RNA参与了多发性骨髓瘤的进展。因此,我们利用小 RNA 测序技术对多发性骨髓瘤、髓外疾病和浆细胞白血病患者骨髓血浆中的这些分子进行了表达谱分析,以确定参与疾病发病机制的新分子。在所分析的亚组中,共有 42 种 microRNA 出现了明显的调控异常。RT-qPCR 的独立验证证实,与髓外疾病和浆细胞白血病患者相比,多发性骨髓瘤患者的 miR-140-3p、miR-584-5p、miR-191-5p 和 miR-143-3p 水平升高。随后的统计分析显示,患者的临床特征或流式细胞仪参数与微RNA表达之间存在明显的相关性。这些结果表明,微RNA的失调可能会导致多发性骨髓瘤的进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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MicroRNA Profiling of Bone Marrow Plasma Extracellular Vesicles in Multiple Myeloma, Extramedullary Disease, and Plasma Cell Leukemia

Multiple myeloma is a plasma cell malignancy characterized by an abnormal increase in monoclonal immunoglobulins. Despite significant advances in treatment, some patients progress to more aggressive forms of multiple myeloma, including extramedullary disease or plasma cell leukemia. Although the exact molecular mechanisms are not known, several studies have confirmed the involvement of small extracellular vesicle-enriched microRNAs in multiple myeloma progression. Therefore, we performed expression profiling of these molecules in bone marrow plasma of multiple myeloma, extramedullary disease, and plasma cell leukemia patients using small RNA sequencing to identify novel molecules involved in disease pathogenesis. In total, 42 microRNAs were significantly dysregulated among analyzed subgroups. Independent validation by RT-qPCR confirmed elevated levels of miR-140-3p, miR-584-5p, miR-191-5p, and miR-143-3p in multiple myeloma patients compared to extramedullary disease and plasma cell leukemia patients. Subsequent statistical analysis revealed significant correlations between patient clinical characteristics or flow cytometry parameters and microRNA expression. These results indicate that dysregulation of microRNAs could contribute to multiple myeloma progression.

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来源期刊
Hematological Oncology
Hematological Oncology 医学-血液学
CiteScore
4.20
自引率
6.10%
发文量
147
审稿时长
>12 weeks
期刊介绍: Hematological Oncology considers for publication articles dealing with experimental and clinical aspects of neoplastic diseases of the hemopoietic and lymphoid systems and relevant related matters. Translational studies applying basic science to clinical issues are particularly welcomed. Manuscripts dealing with the following areas are encouraged: -Clinical practice and management of hematological neoplasia, including: acute and chronic leukemias, malignant lymphomas, myeloproliferative disorders -Diagnostic investigations, including imaging and laboratory assays -Epidemiology, pathology and pathobiology of hematological neoplasia of hematological diseases -Therapeutic issues including Phase 1, 2 or 3 trials as well as allogeneic and autologous stem cell transplantation studies -Aspects of the cell biology, molecular biology, molecular genetics and cytogenetics of normal or diseased hematopoeisis and lymphopoiesis, including stem cells and cytokines and other regulatory systems. Concise, topical review material is welcomed, especially if it makes new concepts and ideas accessible to a wider community. Proposals for review material may be discussed with the Editor-in-Chief. Collections of case material and case reports will be considered only if they have broader scientific or clinical relevance.
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