Antiviral mechanism of Fuzhengjiedu San against porcine reproductive and respiratory syndrome virus

Porcine reproductive and respiratory syndrome virus (PRRSV) is a viral infectious disease that can cause infection in pigs of different ages. The condition known as porcine reproductive and respiratory syndrome poses a serious risk to the world's pig business and results in significant financial losses. Fuzhengjiedu San (FZJDS) is a traditional Chinese medicine compound, the main components include:Radix Isatidis, Radix Astragali and Herba Epimedii. It has been widely used in clinical and experimental studies, showing a wide range of biological activity. However, it is not clear whether FZJDS has anti-PRRSV activity. We observed that FZJDS had significant antiviral activity in Marc-145 cells. And FZJDS could inhibit viral infection in the stages of viral internalization and replication. Furthermore, FZJDS can inhibit PRRSV replication by inhibiting the p53 signaling pathway to affect autophagy, and FZJDS can also inhibit PRRSV replication by inhibiting the PI3K/Akt pathway.We showed in this work that FZJDS inhibits PRRSV replication in vitro and offers a novel therapeutic approach for PRRSV infection.