利用snoKARR-seq在转录组范围内定位snoRNA目标。

IF 3.5 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY ACS Chemical Biology Pub Date : 2025-02-21 Epub Date: 2025-01-14 DOI:10.1021/acschembio.4c00813

Bei Liu

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引用次数: 0

摘要

小核仁RNA （snoRNAs）是一种非编码RNA，主要用于指导RNA的化学修饰，但其更广泛的细胞作用和对人类疾病的贡献仍然难以捉摸。这篇In Focus文章介绍了富含snoRNA的酮醇辅助RNA-RNA测序（snoKARR-seq）的发展，这是一种转录组范围的方法，可以以更高的灵敏度和特异性发现snoRNA靶点。该方法揭示了snoRNAs在蛋白质易位和分泌中的意外作用，扩大了我们对其非规范功能的理解。

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Mapping snoRNA Targets Transcriptome-Wide with snoKARR-seq.

Small nucleolar RNAs (snoRNAs) are noncoding RNAs primarily known for guiding chemical modifications of RNA, but their broader cellular roles and contributions to human diseases remain elusive. This In Focus article introduces the development of snoRNA-enriched kethoxal-assisted RNA-RNA sequencing (snoKARR-seq), a transcriptome-wide approach to uncover snoRNA targets with enhanced sensitivity and specificity. This method revealed an unexpected role for snoRNAs in protein translocation and secretion, expanding our understanding of their noncanonical functions.

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来源期刊

ACS Chemical Biology 生物-生化与分子生物学

CiteScore

7.50

自引率

5.00%

发文量

353

审稿时长

3.3 months

期刊介绍： ACS Chemical Biology provides an international forum for the rapid communication of research that broadly embraces the interface between chemistry and biology. The journal also serves as a forum to facilitate the communication between biologists and chemists that will translate into new research opportunities and discoveries. Results will be published in which molecular reasoning has been used to probe questions through in vitro investigations, cell biological methods, or organismic studies. We welcome mechanistic studies on proteins, nucleic acids, sugars, lipids, and nonbiological polymers. The journal serves a large scientific community, exploring cellular function from both chemical and biological perspectives. It is understood that submitted work is based upon original results and has not been published previously.

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