{"title":"结合Cre-LoxP和单细胞测序技术:洞察细胞外囊泡货物转移。","authors":"Michael W Pfaffl","doi":"10.20517/evcna.2024.58","DOIUrl":null,"url":null,"abstract":"<p><p>The recent study from the Pogge von Strandmann group published in <i>Cellular and Molecular Immunology</i>, by Alashkar Alhamwe <i>et al</i>., combined for the first time the Cre-LoxP recombination system with single-cell sequencing. The group monitored the tumor-derived extracellular vesicle (EV) uptake and the EV functions in the recipient non-malignant cells in a pancreatic ductal adenocarcinoma mouse model. Recombination events and EV uptake, together with resulting gene expression changes in macrophages, neutrophils, and mast cells, were detected by single-cell sequencing technology of the tumor tissue. This new approach is highly specific, as it can identify single EV recipient cells without interfering with the EV biogenesis or the phenotype.</p>","PeriodicalId":520322,"journal":{"name":"Extracellular vesicles and circulating nucleic acids","volume":"5 4","pages":"714-717"},"PeriodicalIF":0.0000,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725425/pdf/","citationCount":"0","resultStr":"{\"title\":\"Combining Cre-LoxP and single-cell sequencing technologies: insights into the extracellular vesicle cargo transfer.\",\"authors\":\"Michael W Pfaffl\",\"doi\":\"10.20517/evcna.2024.58\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The recent study from the Pogge von Strandmann group published in <i>Cellular and Molecular Immunology</i>, by Alashkar Alhamwe <i>et al</i>., combined for the first time the Cre-LoxP recombination system with single-cell sequencing. The group monitored the tumor-derived extracellular vesicle (EV) uptake and the EV functions in the recipient non-malignant cells in a pancreatic ductal adenocarcinoma mouse model. Recombination events and EV uptake, together with resulting gene expression changes in macrophages, neutrophils, and mast cells, were detected by single-cell sequencing technology of the tumor tissue. This new approach is highly specific, as it can identify single EV recipient cells without interfering with the EV biogenesis or the phenotype.</p>\",\"PeriodicalId\":520322,\"journal\":{\"name\":\"Extracellular vesicles and circulating nucleic acids\",\"volume\":\"5 4\",\"pages\":\"714-717\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-11-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725425/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Extracellular vesicles and circulating nucleic acids\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.20517/evcna.2024.58\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Extracellular vesicles and circulating nucleic acids","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20517/evcna.2024.58","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
最近由Alashkar Alhamwe等人发表在《细胞与分子免疫学》上的Pogge von Strandmann小组的研究首次将Cre-LoxP重组系统与单细胞测序结合起来。在胰腺导管腺癌小鼠模型中,研究小组监测了受体非恶性细胞的肿瘤源性细胞外囊泡(EV)摄取和EV功能。通过肿瘤组织单细胞测序技术检测巨噬细胞、中性粒细胞和肥大细胞中重组事件和EV摄取以及由此产生的基因表达变化。这种新方法具有很高的特异性,因为它可以识别单个EV受体细胞,而不会干扰EV的生物发生或表型。
Combining Cre-LoxP and single-cell sequencing technologies: insights into the extracellular vesicle cargo transfer.
The recent study from the Pogge von Strandmann group published in Cellular and Molecular Immunology, by Alashkar Alhamwe et al., combined for the first time the Cre-LoxP recombination system with single-cell sequencing. The group monitored the tumor-derived extracellular vesicle (EV) uptake and the EV functions in the recipient non-malignant cells in a pancreatic ductal adenocarcinoma mouse model. Recombination events and EV uptake, together with resulting gene expression changes in macrophages, neutrophils, and mast cells, were detected by single-cell sequencing technology of the tumor tissue. This new approach is highly specific, as it can identify single EV recipient cells without interfering with the EV biogenesis or the phenotype.