Hesperidin Improves Wound Healing and Mineralization of Periodontal Ligament Cells in Elevated Glucose Conditions.

Introduction: Elevated glucose can have a detrimental effect on the function and healing process of periodontal cells in inflammatory conditions. Hesperidin (HPN), a bioflavonoid found abundantly in citrus fruits, has numerous biological benefits, including regenerative and anti-inflammatory properties. The current in-vitro study aimed to assess the impact of HPN on the proliferation, wound healing, and functionality of periodontal cells in optimal and elevated glucose conditions.

Methods: Human periodontal ligament cells (HPDLCs) were cultured in optimal glucose (1g/L) (OG) and high glucose (4.5 g/L) (HG) conditions. XTT, wound healing, ALP, and calcium release assays were conducted with or without HPN in the culture media.

Results: The statistical analysis revealed that adding different concentrations of HPN (2, 4, 10, or 100 μM) had no significant effect on the viability of HPDLCs under both OG (p=0.436) and HG conditions (p=0.162) compared to the control. However, in the HG condition, the addition of 100 μM HPN resulted in a statistically significant increase in wound closure (p=0.003). Furthermore, in the HG condition, the addition of 100 μM HPN significantly increased ALP activity in the OS- media (p=0.001) and significantly increased calcium release within the OS+ media (p=0.016).

Conclusion: The findings of this study suggest that HPN provides beneficial effects, facilitating repair and mineralization in HPDLCs under HG conditions.