外源性转化生长因子-β1持续增强胎盘组织吲哚胺2,3-双加氧酶表达对复发性妊娠小鼠妊娠结局的影响

IF 2.3 Q2 OBSTETRICS & GYNECOLOGY International Journal of Fertility & Sterility Pub Date : 2025-01-05 DOI:10.22074/ijfs.2024.2011343.1541
Xinghui Wen, Xiaojuan Chen, Mei Li, Jinnian Zhou, Shouli Dao, Shixiang Li, Hui Cheng, Shuyun Zhao, Guanyou Huang
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引用次数: 0

摘要

背景:免疫因素是导致复发性妊娠丢失(RPL)的主要原因,诱导母胎耐受是治疗复发性妊娠丢失的主要方法,但这种方法的效果不确定,需要多次剂量和/或干预。本研究旨在探讨单次给药转化生长因子-β1 (TGF-β1)是否可以改善RPL小鼠的妊娠结局,以及这种改善是否由TGF-β1驱动免疫耐受分子吲哚胺2,3-双加氧酶(IDO)的表达引起。材料和方法:本实验将40只RPL模型小鼠平均分为对照组和治疗组,对照组给予0.01 M的磷酸缓冲盐水(PBS),治疗组通过尾静脉注射含有2、20、200 ng/ml TGF-β1的PBS。在妊娠13.5 d处死小鼠,测定胚胎吸收率。应用western blotting和免疫组化技术检测胎盘组织中IDO、TGF-β1、TGF-β3的表达。结果:在RPL小鼠胎盘组织中IDO的表达与TGF-β1呈正相关(r=0.591, p)结论:外源性TGF-β1改善RPL小鼠妊娠结局,可能的治疗机制是外源性TGF-β1通过相互诱导表达,诱导内源性TGF-β1与IDO持续表达。本实验可能为今后RPL患者的治疗提供新的方向和思路。
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Effect of Exogenous of Transforming Growth Factor-β1 on Pregnancy Outcome in Mice with Recurrent Pregnancy Loss by Persistent Enhancement of Placental Tissue Indoleamine 2,3-Dioxygenase Expression.

Background: The immunologic factors are the chief reason for recurrent pregnancy loss (RPL) and induction of maternal-fetal tolerance is the main treatment for this cause of RPL, but the effect of this method is uncertainly and needs multiple doses and/or interventions. The aim of this study was to investigate whether a single administration of transforming growth factor-β1 (TGF-β1) can improve the pregnancy outcomes of RPL mice and whether the improvement is cause by TGF-β1 driving the expression of immune tolerance molecule indoleamine 2,3-dioxygenase (IDO).

Materials and methods: In this experimental study, 40 RPL model mice were equally divided into a control group, that received 0.01 M phosphate-buffered saline (PBS), and a treatment group, that received PBS containing 2, 20, and 200 ng/ml TGF-β1 via tail vein injection. The mice were sacrificed at 13.5 days of pregnancy and the embryo resorption rate was determined. The expression of IDO, TGF-β1, and TGF-β3 were detected in the placenta using western blotting and immunohistochemistry techniques.

Results: The expression of IDO was positively correlated with TGF-β1 in the placental tissue of RPL mice (r=0.591, P<0.001). In all treatment groups, the embryo resorption rates were significantly lower than the control group and the expression of IDO in the placental tissue of all treatment groups was significantly higher than the control group. The expression of TGF-β1 increased gradually from, 2, 20 to 200 ng/ml in treatment groups, and the concentration of exogenous TGF-β1 positively correlated with the expression of TGF-β1, in placental tissues in treatment groups (r=0.372, P=0.018).

Conclusion: Exogenous TGF-β1 improves pregnancy outcomes in RPL mice, and the possible therapeutic mechanism is that exogenous TGF-β1 induces the persistent expression of endogenous TGF-β1 and IDO due to mutually induced expression of the other. This experiment may provide a new direction and idea for the future treatment of RPL patients.

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来源期刊
CiteScore
4.20
自引率
0.00%
发文量
68
审稿时长
>12 weeks
期刊介绍: International Journal of Fertility & Sterility is a quarterly English publication of Royan Institute . The aim of the journal is to disseminate information through publishing the most recent scientific research studies on Fertility and Sterility and other related topics. Int J Fertil Steril has been certified by Ministry of Culture and Islamic Guidance in 2007 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2008. Int J Fertil Steril is an Open Access journal.
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