Walid Anwar, Abdulsalam M Kassem, Ayman Salama, Mohamed F Zidan, Ahmed H Ibrahim, Ibrahim A Elbahwy, Elsaied H Barakat, Tarek M Faris, Maged K Elsayad, Ahmed M Samy, Mahmoud M A Elsayed, Abdelaziz E Abdelaziz
{"title":"利用表面修饰的纳米结构脂质载体优化阿苯达唑在肝细胞癌（HepG2细胞）中的递送和抗癌潜力评估。","authors":"Walid Anwar, Abdulsalam M Kassem, Ayman Salama, Mohamed F Zidan, Ahmed H Ibrahim, Ibrahim A Elbahwy, Elsaied H Barakat, Tarek M Faris, Maged K Elsayad, Ahmed M Samy, Mahmoud M A Elsayed, Abdelaziz E Abdelaziz","doi":"10.1080/02652048.2025.2451848","DOIUrl":null,"url":null,"abstract":"<p><p>This study evaluated albendazole (ABZ) nanostructured lipid carriers (NLCs) for hepatocellular carcinoma treatment. ABZ-NLCs were prepared using emulsification-ultrasonication and optimised using a Box-Behnken design. Independent variables-lipids concentration (X<sub>1</sub>), surfactant concentration (X<sub>2</sub>), and sonication duration (X<sub>3</sub>)-were assessed for their effect on mean diameter (Y<sub>1</sub>), PDI (Y<sub>2</sub>), and entrapment efficiency (Y<sub>3</sub>). The optimised formulation exhibited a mean diameter of 166.13 ± 3.72 nm, a PDI of 0.17 ± 0.01, a zeta potential of -39.86 ± 1.84 mV, an entrapment efficiency of 94.25 ± 6.12%, and a loading capacity of 99.93 ± 7.15 mg/g. Following chitosan coating (ABZ-CS-NLCs), all parameters were maintained, and the zeta potential developed to +24.61 ± 1.32 mV, improving cellular interaction. The cytotoxicity assays revealed that ABZ-CS-NLCs were more effective than uncoated NLCs and free ABZ, with an IC50 value of 8.89 μM in HepG2 cells. Overall, ABZ-CS-NLCs demonstrate a promising and effective delivery platform for targeted hepatic cancer therapy.</p>","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"1-16"},"PeriodicalIF":3.0000,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optimisation of albendazole delivery and assessment of anticancer potential in hepatocellular carcinoma (HepG2 cells) using surface modified nanostructured lipid carriers.\",\"authors\":\"Walid Anwar, Abdulsalam M Kassem, Ayman Salama, Mohamed F Zidan, Ahmed H Ibrahim, Ibrahim A Elbahwy, Elsaied H Barakat, Tarek M Faris, Maged K Elsayad, Ahmed M Samy, Mahmoud M A Elsayed, Abdelaziz E Abdelaziz\",\"doi\":\"10.1080/02652048.2025.2451848\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study evaluated albendazole (ABZ) nanostructured lipid carriers (NLCs) for hepatocellular carcinoma treatment. ABZ-NLCs were prepared using emulsification-ultrasonication and optimised using a Box-Behnken design. Independent variables-lipids concentration (X<sub>1</sub>), surfactant concentration (X<sub>2</sub>), and sonication duration (X<sub>3</sub>)-were assessed for their effect on mean diameter (Y<sub>1</sub>), PDI (Y<sub>2</sub>), and entrapment efficiency (Y<sub>3</sub>). The optimised formulation exhibited a mean diameter of 166.13 ± 3.72 nm, a PDI of 0.17 ± 0.01, a zeta potential of -39.86 ± 1.84 mV, an entrapment efficiency of 94.25 ± 6.12%, and a loading capacity of 99.93 ± 7.15 mg/g. Following chitosan coating (ABZ-CS-NLCs), all parameters were maintained, and the zeta potential developed to +24.61 ± 1.32 mV, improving cellular interaction. The cytotoxicity assays revealed that ABZ-CS-NLCs were more effective than uncoated NLCs and free ABZ, with an IC50 value of 8.89 μM in HepG2 cells. Overall, ABZ-CS-NLCs demonstrate a promising and effective delivery platform for targeted hepatic cancer therapy.</p>\",\"PeriodicalId\":16391,\"journal\":{\"name\":\"Journal of microencapsulation\",\"volume\":\" \",\"pages\":\"1-16\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-01-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of microencapsulation\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/02652048.2025.2451848\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microencapsulation","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/02652048.2025.2451848","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Optimisation of albendazole delivery and assessment of anticancer potential in hepatocellular carcinoma (HepG2 cells) using surface modified nanostructured lipid carriers.
This study evaluated albendazole (ABZ) nanostructured lipid carriers (NLCs) for hepatocellular carcinoma treatment. ABZ-NLCs were prepared using emulsification-ultrasonication and optimised using a Box-Behnken design. Independent variables-lipids concentration (X1), surfactant concentration (X2), and sonication duration (X3)-were assessed for their effect on mean diameter (Y1), PDI (Y2), and entrapment efficiency (Y3). The optimised formulation exhibited a mean diameter of 166.13 ± 3.72 nm, a PDI of 0.17 ± 0.01, a zeta potential of -39.86 ± 1.84 mV, an entrapment efficiency of 94.25 ± 6.12%, and a loading capacity of 99.93 ± 7.15 mg/g. Following chitosan coating (ABZ-CS-NLCs), all parameters were maintained, and the zeta potential developed to +24.61 ± 1.32 mV, improving cellular interaction. The cytotoxicity assays revealed that ABZ-CS-NLCs were more effective than uncoated NLCs and free ABZ, with an IC50 value of 8.89 μM in HepG2 cells. Overall, ABZ-CS-NLCs demonstrate a promising and effective delivery platform for targeted hepatic cancer therapy.
期刊介绍：
The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation.
The journal covers:
Chemistry of encapsulation materials
Physics of release through the capsule wall and/or desorption from carrier
Techniques of preparation, content and storage
Many uses to which microcapsules are put.
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