饲料中添加乳酸乳球菌Z-2对鲤幼鱼生长、寄主健康及对嗜水气单胞菌抗性的影响

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2025-01-20 DOI:10.1002/jsfa.14136

Junchang Feng, Xiao Yuan, Shasha Liu, Xiaobei Liu, Wenshan Cui, Shikun Feng, Jianxin Zhang

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引用次数: 0

摘要

背景：乳酸乳球菌Z-2先前从鲤鱼肠中分离得到。为研究乳酸菌Z-2的最佳剂量对鲤鱼生长、寄主健康和对嗜水气单胞菌的抗性的影响，采用无CK组和CK+组和添加5 × 107 （A组）、5 × 108 （B组）和5 × 109 CFU g-1 （C组）5种试验饲料进行了8周的乳酸菌Z-2试验。结果：添加乳酸菌Z-2可提高幼鱼的末重、增重、特定生长率、条件因子和肌肉粗蛋白质含量，但饲料系数、肌肉粗脂肪和粗灰分含量均低于CK组(P)。结论：饲粮中添加乳酸菌Z-2可改善幼鱼的生长性能、免疫反应、抗氧化能力、肠道健康和抗病性。最终推荐在饲料中添加5 × 108 CFU g-1的饲粮L -乳酸Z-2。©2025化学工业协会。

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Effects of dietary Lactococcus lactis Z-2 on growth, host health and resistance to Aeromonas hydrophila in juvenile common carp (Cyprinus carpio L.).

Background: Lactococcus lactis Z-2 was previously isolated from common carp intestine. In order to investigate the effects of optimal dose of L. lactis Z-2 on growth, host health and disease resistance to Aeromonas hydrophila in common carp, five experimental diets, including without (CK and CK+ groups) or with 5 × 10⁷ (group A), 5 × 10⁸ (group B) and 5 × 10⁹ CFU g^-1 (group C) L. lactis Z-2, were evaluated for 8 weeks.

Results: Supplementation of L. lactis Z-2 increased final weights, weight gain, specific growth rate, condition factor and muscle crude protein content, while feed conversion ratio, muscle crude lipid and ash contents were lower than in the CK group (P < 0.05). Furthermore, L. lactis Z-2 also promoted intestinal digestive enzyme (lipase, amylase, protease) activities, improved intestinal morphology and upregulated gene (ZO-1, Occludin, Claudin) expression of intestinal barrier; stimulated secretion of proinflammatory (IL-6, IL-1β, TNF-α) and anti-inflammatory cytokines (TGF-β, IL-10) in serum; and boosted AKP, ACP, LZM and antioxidant enzyme (T-AOC, GSH-Px, T-SOD, CAT) activities of hepatopancreas (P < 0.05). Among them, the effect of group B was more prominent. After A. hydrophila infection, the CK+ group showed overexpression of immune indicators and decreased antioxidant capacity. Whereas dietary intervention with L. lactis Z-2 significantly reversed these changes. Moreover, the survival rate was significantly higher (100%) in group B than in the CK+ group (36.36%) after A. hydrophila infection (P < 0.05).

Conclusion: Dietary L. lactis Z-2 improved the growth performance, immune response, antioxidant capacity, intestinal health and disease resistance of juvenile common carp. Ultimately, 5 × 10⁸ CFU g^-1 dietary L lactis Z-2 is recommended for the formulation of feed. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.