苦荞采前发芽鉴定及数量性状位点定位。

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2025-01-17 DOI:10.1002/jsfa.14117

Liwei Zhu, Yong Lv, Taoxiong Shi, Juan Huang, Qianqian Du, Guohong Tang, Genlou Sun, Odika Prince, Qingfu Chen

{"title":"苦荞采前发芽鉴定及数量性状位点定位。","authors":"Liwei Zhu, Yong Lv, Taoxiong Shi, Juan Huang, Qianqian Du, Guohong Tang, Genlou Sun, Odika Prince, Qingfu Chen","doi":"10.1002/jsfa.14117","DOIUrl":null,"url":null,"abstract":"Background: Tartary buckwheat (Fagopyrum tartaricum) is particularly vulnerable to pre-harvest sprouting (PHS) due to its extended flowering and fruiting cycle, especially during periods of prolonged rainfall. This susceptibility has significant adverse effects on yield, quality and post-harvest processing. In this study, a recombinant inbred lines (RILs) population (XJ-RILs) was developed from a cross between the PHS-susceptible Tartary buckwheat variety 'Xiaomiqiao' (female parent) and the highly PHS-resistant variety 'Jinqiaomai 2' (male parent). Key traits, including germination percentage, germination energy, germination index, field PHS (PHS-F) and simulated PHS (PHS-S), were evaluated, and a quantitative trait locus (QTL) mapping analysis was performed.Results: (i) PHS-S was strongly and significantly correlated with PHS-F. (ii) A total of 11 QTLs associated with seed germination and 14 QTLs related to PHS were identified. Notably, the major QTL cluster qPHS8-1 was consistently detected and mapped within the interval of 8.53-9.65 Mbp on chromosome Ft8. (iii) Genotyping of 221 XJ-RILs across eight chromosomes revealed five residual heterozygous lines carrying a heterozygous interval of qPHS8-1 cluster, with inbred line R56 being particularly suited for the fine mapping of qPHS8-1.Conclusion: The PHS-S test, conducted on entire Tartary buckwheat spikes, is an effective and comprehensive method for assessing PHS resistance in this crop. QTL mapping identified qPHS8-1 as a major locus for PHS resistance, and inbred line R56 offers a promising resource for further fine mapping of this cluster. © 2025 Society of Chemical Industry.","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification and quantitative trait locus mapping of Tartary buckwheat pre-harvest sprouting.\",\"authors\":\"Liwei Zhu, Yong Lv, Taoxiong Shi, Juan Huang, Qianqian Du, Guohong Tang, Genlou Sun, Odika Prince, Qingfu Chen\",\"doi\":\"10.1002/jsfa.14117\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Tartary buckwheat (Fagopyrum tartaricum) is particularly vulnerable to pre-harvest sprouting (PHS) due to its extended flowering and fruiting cycle, especially during periods of prolonged rainfall. This susceptibility has significant adverse effects on yield, quality and post-harvest processing. In this study, a recombinant inbred lines (RILs) population (XJ-RILs) was developed from a cross between the PHS-susceptible Tartary buckwheat variety 'Xiaomiqiao' (female parent) and the highly PHS-resistant variety 'Jinqiaomai 2' (male parent). Key traits, including germination percentage, germination energy, germination index, field PHS (PHS-F) and simulated PHS (PHS-S), were evaluated, and a quantitative trait locus (QTL) mapping analysis was performed.Results: (i) PHS-S was strongly and significantly correlated with PHS-F. (ii) A total of 11 QTLs associated with seed germination and 14 QTLs related to PHS were identified. Notably, the major QTL cluster qPHS8-1 was consistently detected and mapped within the interval of 8.53-9.65 Mbp on chromosome Ft8. (iii) Genotyping of 221 XJ-RILs across eight chromosomes revealed five residual heterozygous lines carrying a heterozygous interval of qPHS8-1 cluster, with inbred line R56 being particularly suited for the fine mapping of qPHS8-1.Conclusion: The PHS-S test, conducted on entire Tartary buckwheat spikes, is an effective and comprehensive method for assessing PHS resistance in this crop. QTL mapping identified qPHS8-1 as a major locus for PHS resistance, and inbred line R56 offers a promising resource for further fine mapping of this cluster. © 2025 Society of Chemical Industry.\",\"PeriodicalId\":17725,\"journal\":{\"name\":\"Journal of the Science of Food and Agriculture\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2025-01-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Science of Food and Agriculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1002/jsfa.14117\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Science of Food and Agriculture","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/jsfa.14117","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

摘要

背景：苦荞（Fagopyrum tartaricum）特别容易受到收获前发芽（PHS）的影响，因为它的开花和结果周期延长，特别是在长时间降雨期间。这种敏感性对产量、品质和采后加工都有显著的不利影响。本研究利用对稻瘟病敏感的苦荞品种“小米翘”（母本）与稻瘟病抗性强的品种“金乔麦2号”（父本）杂交，构建了重组自交系（RILs）群体XJ-RILs。对发芽率、萌发能、萌发指数、田间小灵通（PHS- f）和模拟小灵通（PHS- s）等关键性状进行评价，并进行数量性状位点（QTL）定位分析。结果：(1)PHS-S与PHS-F呈显著强相关。（ii）共鉴定出11个与种子萌发相关的qtl和14个与小灵通相关的qtl。值得注意的是，主要QTL集群qPHS8-1在Ft8染色体8.53-9.65 Mbp的区间内被一致检测和定位。（iii）对8条染色体上221个xj - ril进行基因分型，发现5个残留杂合系携带qPHS8-1簇杂合区间，其中自交系R56特别适合qPHS8-1的精细定位。结论：对苦荞全穗进行PHS- s试验，是评价苦荞小灵通抗性的有效、综合的方法。QTL定位鉴定出qPHS8-1是小灵通抗性的主要位点，自交系R56为该群体的进一步精细定位提供了良好的资源。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Identification and quantitative trait locus mapping of Tartary buckwheat pre-harvest sprouting.

Background: Tartary buckwheat (Fagopyrum tartaricum) is particularly vulnerable to pre-harvest sprouting (PHS) due to its extended flowering and fruiting cycle, especially during periods of prolonged rainfall. This susceptibility has significant adverse effects on yield, quality and post-harvest processing. In this study, a recombinant inbred lines (RILs) population (XJ-RILs) was developed from a cross between the PHS-susceptible Tartary buckwheat variety 'Xiaomiqiao' (female parent) and the highly PHS-resistant variety 'Jinqiaomai 2' (male parent). Key traits, including germination percentage, germination energy, germination index, field PHS (PHS-F) and simulated PHS (PHS-S), were evaluated, and a quantitative trait locus (QTL) mapping analysis was performed.

Results: (i) PHS-S was strongly and significantly correlated with PHS-F. (ii) A total of 11 QTLs associated with seed germination and 14 QTLs related to PHS were identified. Notably, the major QTL cluster qPHS8-1 was consistently detected and mapped within the interval of 8.53-9.65 Mbp on chromosome Ft8. (iii) Genotyping of 221 XJ-RILs across eight chromosomes revealed five residual heterozygous lines carrying a heterozygous interval of qPHS8-1 cluster, with inbred line R56 being particularly suited for the fine mapping of qPHS8-1.

Conclusion: The PHS-S test, conducted on entire Tartary buckwheat spikes, is an effective and comprehensive method for assessing PHS resistance in this crop. QTL mapping identified qPHS8-1 as a major locus for PHS resistance, and inbred line R56 offers a promising resource for further fine mapping of this cluster. © 2025 Society of Chemical Industry.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.