ads -17负载的南极念珠菌脂肪酶B通过油酸和甘油酯化选择性合成三酰基甘油。

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2025-01-21 DOI:10.1002/jsfa.14137

Jiawei Zheng, Qiangyue Zhang, Nanjing Zhong

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引用次数: 0

摘要

背景：具有高活性和高选择性的固定化酶在实际应用中具有重要意义。本研究将南极念珠菌脂肪酶B （CALB）固定在大孔树脂ADS-17上，通过油酸和甘油的酯化反应合成三酰基甘油（TAG）。通过单因素试验和正交试验对反应条件进行优化，并对固定化CALB （CALB@ADS-17）的可重复使用性进行评价。此外，还研究了脂肪酶固定化的机理，探讨了CALB@ADS-17的催化机理。结果：在最佳工艺条件下，油酸转化率达99.20%，TAG含量为91.58 wt%。此外，CALB@ADS-17在重复使用11个周期后，保留了其初始活性的84.28%。脂肪酶固定化的机理是疏水吸附。温度与油酸转化率的关系为lnV0 = 6.3316 ~ 4.3321/T，活化能（Ea）为36.02 kJ mol-1。CALB@ADS-17未表现出明显的界面活化现象。其动力学参数vmax、kcat、Km、Ki和kcat/Km分别为0.01265 μmol L-1 s-1、9310.72 s-1、0.4907 mmol L-1、3.997 mmol L-1和1.90 × 104 L mmol-1 s-1，符合Michaelis-Menten模型。结论：CALB@ADS-17具有良好的酯化性能，对TAG的生成具有良好的选择性。此外，CALB@ADS-17在酯化反应中表现出良好的可重复使用性，具有实际应用潜力。©2025化学工业协会。

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Selective synthesis of triacylglycerols by the ADS-17-supported Candida antarctica lipase B through esterification of oleic acid and glycerol

BACKGROUND

Immobilized enzyme possessing both high activity and good selectivity is important in practice. In this study, Candida antarctica lipase B (CALB) was immobilized onto the macroporous resin ADS-17 for triacylglycerol (TAG) synthesis through esterification of oleic acid and glycerol. The reaction conditions were optimized by single-factor study and orthogonal test, and the reusability of the immobilized CALB (CALB@ADS-17) was evaluated. In addition, the mechanism of lipase immobilization was studied and the catalytic mechanism of CALB@ADS-17 was investigated.

RESULTS

Oleic acid conversion up to 99.20% and TAG content at 91.58 wt% could be obtained under optimal conditions. In addition, the CALB@ADS-17 retained 84.28% of its initial activity after 11 cycles of reuse. The mechanism of lipase immobilization was through hydrophobic adsorption. The relationship between temperature and oleic acid conversion was lnV₀ = 6.3316 − 4.3321/T, and the activation energy (E_a) was 36.02 kJ mol⁻¹. CALB@ADS-17 did not exhibit an obvious interfacial activation phenomenon. Its kinetic behavior can be described by the Michaelis–Menten model, whose kinetic parameters of v_max, k_cat, K_m, K_i, and k_cat/K_m were 0.01265 μmol L⁻¹ s⁻¹, 9310.72 s⁻¹, 0.4907 mmol L⁻¹, 3.997 mmol L⁻¹, and 1.90 × 10⁴ L mmol⁻¹ s⁻¹, respectively.

CONCLUSION

CALB@ADS-17 showed good esterification performance and exhibited good selectivity towards TAG generation. In addition, CALB@ADS-17 exhibited good reusability in esterification reactions and has potential in practical applications. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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