绘制温室-马铃薯蚜虫(Aulacorthum solani)基因组序列。

IF 2.4 3区 生物学 Q3 GENETICS & HEREDITY G3: Genes|Genomes|Genetics Pub Date : 2025-03-18 DOI:10.1093/g3journal/jkaf013
Joseph Torres, Paula Rozo-Lopez, William Brewer, Omid Saleh Ziabari, Benjamin J Parker
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引用次数: 0

摘要

茄蚜(Aulacorthum solani)是一种世界性的农业害虫蚜虫,能以多种寄主植物为食。这种昆虫是植物病毒的载体,对作物造成伤害,包括韧皮部的损失导致生长迟缓。我们发现可公开获得的茄茄蚜基因组被另一种蚜虫污染,我们使用条形码等基因实验室系产生了一个新的基因组。我们生成了Oxford Nanopore和Illumina reads来组装一个基因组草图,我们对RNA进行测序,以帮助对我们的组装进行注释。我们的茄茄基因组全长671mbp,包含7020个contigs, N50长度为196kb, BUSCO完整性为98.6%。在E-GAPx预测的24,981个基因中,22,804个被注释了基于与其他蚜虫物种同源性的推测功能。该基因组将为从农业和基因组学角度研究蚜虫的研究人员提供有用的资源。
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Draft genome sequence of the glasshouse-potato aphid Aulacorthum solani.

Aulacorthum solani is a worldwide agricultural pest aphid capable of feeding on a wide range of host plants. This insect is a vector of plant viruses and causes injury to crops including stunted growth from the loss of phloem. We found that the publicly available genome for A. solani is contaminated with another aphid species, and we produced a new genome using a barcoded isogenic laboratory line. We generated Oxford Nanopore and Illumina reads to assemble a draft genome, and we sequenced RNA to aid in the annotation of our assembly. Our A. solani genome is 671 Mb containing 7,020 contigs with an N50 length of 196 kb with a BUSCO completeness of 98.6%. Out of the 24,981 genes predicted by EGAPx, 22,804 were annotated with putative functions based on homology to other aphid species. This genome will provide a useful resource for the community of researchers studying aphids from agricultural and genomic perspectives.

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来源期刊
G3: Genes|Genomes|Genetics
G3: Genes|Genomes|Genetics GENETICS & HEREDITY-
CiteScore
5.10
自引率
3.80%
发文量
305
审稿时长
3-8 weeks
期刊介绍: G3: Genes, Genomes, Genetics provides a forum for the publication of high‐quality foundational research, particularly research that generates useful genetic and genomic information such as genome maps, single gene studies, genome‐wide association and QTL studies, as well as genome reports, mutant screens, and advances in methods and technology. The Editorial Board of G3 believes that rapid dissemination of these data is the necessary foundation for analysis that leads to mechanistic insights. G3, published by the Genetics Society of America, meets the critical and growing need of the genetics community for rapid review and publication of important results in all areas of genetics. G3 offers the opportunity to publish the puzzling finding or to present unpublished results that may not have been submitted for review and publication due to a perceived lack of a potential high-impact finding. G3 has earned the DOAJ Seal, which is a mark of certification for open access journals, awarded by DOAJ to journals that achieve a high level of openness, adhere to Best Practice and high publishing standards.
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