肠上皮中两亲肽自组装形成的番茄红素纳米胶束的细胞摄取和运输机制。

IF 4 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2025-01-24 DOI:10.1002/jsfa.14151

Lijun Su, Zhipeng Yu, Wenzhu Zhao

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引用次数: 0

摘要

背景：本研究旨在阐明番茄红素纳米胶束的转运机制，以促进肠道对番茄红素的吸收。采用等温滴定量热法（ITC）研究了番茄红素与纳米胶束的相互作用机理。利用Caco-2细胞模型研究了番茄红素负载纳米胶束的细胞毒性、细胞摄取、内吞作用和细胞内运输途径。结果：ITC结果表明，纳米胶束与番茄红素的结合是一个熵驱动的自发放热反应，疏水相互作用是主要驱动力。负载番茄红素的纳米胶束没有细胞毒性，纳米胶囊化后Caco-2细胞对番茄红素的摄取增加了2.20倍。番茄红素纳米胶束的细胞内运输结果表明，内质网、高尔基体和溶酶体在这一过程中起关键作用。细胞内运输结果表明，内质网、高尔基体和溶酶体是番茄红素纳米胶束细胞内运输的重要细胞器。结论：纳米胶束能有效促进番茄红素的细胞吸收，是一种很有前景的番茄红素递送载体。本研究有助于理解纳米胶束在肠上皮细胞中的转运机制。©2025化学工业协会。

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Cellular uptake and transport mechanism of lycopene-loaded nanomicelles formed by amphiphilic peptide self-assembly in the intestinal epithelium

BACKGROUND

This study aimed to elucidate the transport mechanism of lycopene-loaded nanomicelles to improve intestinal absorption of lycopene. The interactive mechanism between lycopene and nanomicelles was investigated through isothermal titration calorimetry (ITC). The cytotoxicity, cellular uptake, endocytosis, and intracellular transport pathways of lycopene-loaded nanomicelles were investigated using the Caco-2 cell model.

RESULTS

The ITC results demonstrated that nanomicelles/lycopene binding was an entropy-driven spontaneous exothermic reaction, and hydrophobic interactions were the main driving force. Lycopene-loaded nanomicelles were not cytotoxic, and uptake of lycopene by Caco-2 cells increased 2.20-fold after nanoencapsulation. The results of intracellular transport of lycopene-loaded nanomicelles indicated that the endoplasmic reticulum, Golgi apparatus, and lysosomes play key roles in this process. The intracellular transport results showed that the endoplasmic reticulum, Golgi apparatus, and lysosomes were important organelles for intracellular transport of lycopene-loaded nanomicelles.

CONCLUSION

Nanomicelles effectively enhance the cellular uptake of lycopene and are promising carriers for its delivery. This study contributes to the understanding of the transport mechanism of nanomicelles through intestinal epithelial cells. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.