Young Wha Koh, Jae-Ho Han, Seokjin Haam, Hyun Woo Lee
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引用次数: 0
摘要
背景/目的：脑转移（BMs）在肺鳞癌（LUSC）中十分罕见。因此，有关可用于预测脑转移的生物标志物或机制的研究十分有限。为了验证 mRNA 图谱能否准确预测 BMs，我们在 20 份 TNM 匹配的 LUSC 组织样本上使用了机器学习方法：我们进行了通路和免疫组化分析，以研究BM的内在机制：结果：共鉴定出15个与BM相关的mRNA。15个mRNA特征在预测BM方面的准确率很高，曲线下面积和准确率分别为0.940和0.9。通路分析表明，免疫相关通路（白细胞跨内皮迁移、Fc γ R 介导的吞噬作用和自然杀伤细胞介导的细胞毒性）受到抑制，这表明免疫抑制状态可能与 BM 的发生有关。在通过免疫组化染色确认的验证组中，BM 组的 CD4+ T 细胞或 CD8+ T 细胞水平明显低于无 BM 组。LUSC患者的BM与免疫抑制状态有关：结论：免疫疗法可有效预防 LUSC 患者的 BM。
Immunosuppressive State May Lead to Brain Metastases in Lung Squamous Cell Carcinoma: Gene Expression and Immunohistochemical Analysis.
Background/aim: Brain metastases (BMs) are rare in lung squamous cell carcinoma (LUSC). Therefore, research on biomarkers or mechanisms that can be used to predict them is limited. To verify whether mRNA profiles can accurately predict BMs, we used a machine learning approach on 20 TNM-matched LUSC tissue samples.
Materials and methods: We conducted pathway and immunohistochemical analyses to investigate the underlying mechanisms of BM.
Results: A total of 15 mRNAs linked to BM were identified. The 15-mRNA signature was highly accurate in predicting BM, with an area under the curve and accuracy of 0.940 and 0.9, respectively. The pathway analysis revealed that immune-related pathways (leukocyte transendothelial migration, Fc gamma R-mediated phagocytosis, and natural killer cell-mediated cytotoxicity) were suppressed, suggesting that an immunosuppressive state may be involved in the development of BM. In the validation set confirmed by immunohistochemical staining, the BM group exhibited significantly lower levels of CD4+ T cells or CD8+ T cells than the group without BM. BM in patients with LUSC was associated with an immunosuppressive state.
Conclusion: Immunotherapy may be effective in preventing BM in patients with LUSC.
期刊介绍：
ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed.
ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies).
Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.
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