Mada Triandala Sibero, Zhou-Qing Zheng, Fei-Na Li, Shao-Wei Liu, Xin Xiang, Li Tuo, Cheng-Hang Sun
{"title":"红树Tersicoccus mangrovi sp. nov.，一种来自三宝垄市红树林沉积物的新型放线菌。","authors":"Mada Triandala Sibero, Zhou-Qing Zheng, Fei-Na Li, Shao-Wei Liu, Xin Xiang, Li Tuo, Cheng-Hang Sun","doi":"10.1099/ijsem.0.006669","DOIUrl":null,"url":null,"abstract":"<p><p>A Gram-stain-positive, aerobic and non-motile actinobacterium, designated strain MR15.9<sup>T</sup>, was isolated from sediment collected from a mangrove ecosystem in Semarang city, Indonesia. Strain MR15.9<sup>T</sup> grew at 4-37 °C (optimum 30 °C), pH 6.0-11.0 (optimum 7.0-8.0), with 0-10% (w/v) NaCl (optimum 0-3%). The genome of strain MR15.9<sup>T</sup> was 3.67 Mbp with 71.7 mol% G+C content. Phylogenetic analysis based on 16S rRNA gene sequence and genome sequence directed that strain MR15.9<sup>T</sup> formed a well-supported clade with <i>Tersicoccus solisilvae</i> CGMCC 1.15480<sup>T</sup> and <i>Tersicoccus phoenicis</i> 1P05MA<sup>T</sup> and shared the highest similarity to <i>T. solisilvae</i> CGMCC 1.15480<sup>T</sup> (98.9% sequence similarity) and <i>T. phoenicis</i> 1P05MA<sup>T</sup> (98.6% sequence similarity). However, the comparative genome analysis between strain MR15.9<sup>T</sup> and <i>T. solisilvae</i> CGMCC 1.15480<sup>T</sup> gave average nt identity value of 85.2% and digital DNA-DNA hybridization value of 29.0%, which were below the statistical threshold for the delineation of the species. Chemotaxonomic data showed that the major fatty acids were anteiso-C<sub>15:0</sub>, anteiso-C<sub>17:0</sub> and iso-C<sub>16:0</sub>. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids. MK-8(H<sub>2</sub>) and MK-9(H<sub>2</sub>) were noted as the predominant respiratory quinones in this strain. The results of polyphasic characterization indicated that strain MR15.9<sup>T</sup> represents a novel species of the genus <i>Tersicoccus</i>. The name <i>Tersicoccus mangrovi</i> sp. nov. is proposed, with the type strain MR15.9<sup>T</sup> (=MCCC 1K08875<sup>T</sup>=KCTC 59105<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Tersicoccus mangrovi</i> sp. nov., a novel actinobacterium from mangrove sediment in Semarang city, Indonesia.\",\"authors\":\"Mada Triandala Sibero, Zhou-Qing Zheng, Fei-Na Li, Shao-Wei Liu, Xin Xiang, Li Tuo, Cheng-Hang Sun\",\"doi\":\"10.1099/ijsem.0.006669\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A Gram-stain-positive, aerobic and non-motile actinobacterium, designated strain MR15.9<sup>T</sup>, was isolated from sediment collected from a mangrove ecosystem in Semarang city, Indonesia. Strain MR15.9<sup>T</sup> grew at 4-37 °C (optimum 30 °C), pH 6.0-11.0 (optimum 7.0-8.0), with 0-10% (w/v) NaCl (optimum 0-3%). The genome of strain MR15.9<sup>T</sup> was 3.67 Mbp with 71.7 mol% G+C content. Phylogenetic analysis based on 16S rRNA gene sequence and genome sequence directed that strain MR15.9<sup>T</sup> formed a well-supported clade with <i>Tersicoccus solisilvae</i> CGMCC 1.15480<sup>T</sup> and <i>Tersicoccus phoenicis</i> 1P05MA<sup>T</sup> and shared the highest similarity to <i>T. solisilvae</i> CGMCC 1.15480<sup>T</sup> (98.9% sequence similarity) and <i>T. phoenicis</i> 1P05MA<sup>T</sup> (98.6% sequence similarity). However, the comparative genome analysis between strain MR15.9<sup>T</sup> and <i>T. solisilvae</i> CGMCC 1.15480<sup>T</sup> gave average nt identity value of 85.2% and digital DNA-DNA hybridization value of 29.0%, which were below the statistical threshold for the delineation of the species. Chemotaxonomic data showed that the major fatty acids were anteiso-C<sub>15:0</sub>, anteiso-C<sub>17:0</sub> and iso-C<sub>16:0</sub>. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids. MK-8(H<sub>2</sub>) and MK-9(H<sub>2</sub>) were noted as the predominant respiratory quinones in this strain. The results of polyphasic characterization indicated that strain MR15.9<sup>T</sup> represents a novel species of the genus <i>Tersicoccus</i>. The name <i>Tersicoccus mangrovi</i> sp. nov. is proposed, with the type strain MR15.9<sup>T</sup> (=MCCC 1K08875<sup>T</sup>=KCTC 59105<sup>T</sup>).</p>\",\"PeriodicalId\":14390,\"journal\":{\"name\":\"International journal of systematic and evolutionary microbiology\",\"volume\":\"75 2\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of systematic and evolutionary microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/ijsem.0.006669\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006669","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Tersicoccus mangrovi sp. nov., a novel actinobacterium from mangrove sediment in Semarang city, Indonesia.
A Gram-stain-positive, aerobic and non-motile actinobacterium, designated strain MR15.9T, was isolated from sediment collected from a mangrove ecosystem in Semarang city, Indonesia. Strain MR15.9T grew at 4-37 °C (optimum 30 °C), pH 6.0-11.0 (optimum 7.0-8.0), with 0-10% (w/v) NaCl (optimum 0-3%). The genome of strain MR15.9T was 3.67 Mbp with 71.7 mol% G+C content. Phylogenetic analysis based on 16S rRNA gene sequence and genome sequence directed that strain MR15.9T formed a well-supported clade with Tersicoccus solisilvae CGMCC 1.15480T and Tersicoccus phoenicis 1P05MAT and shared the highest similarity to T. solisilvae CGMCC 1.15480T (98.9% sequence similarity) and T. phoenicis 1P05MAT (98.6% sequence similarity). However, the comparative genome analysis between strain MR15.9T and T. solisilvae CGMCC 1.15480T gave average nt identity value of 85.2% and digital DNA-DNA hybridization value of 29.0%, which were below the statistical threshold for the delineation of the species. Chemotaxonomic data showed that the major fatty acids were anteiso-C15:0, anteiso-C17:0 and iso-C16:0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids. MK-8(H2) and MK-9(H2) were noted as the predominant respiratory quinones in this strain. The results of polyphasic characterization indicated that strain MR15.9T represents a novel species of the genus Tersicoccus. The name Tersicoccus mangrovi sp. nov. is proposed, with the type strain MR15.9T (=MCCC 1K08875T=KCTC 59105T).
期刊介绍：
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.
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