利用结构转换适体与DNA测序相结合来定量代谢物

IF 41.7 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Nature biotechnology Pub Date : 2025-02-04 DOI:10.1038/s41587-025-02554-7

June H. Tan, Andrew G. Fraser

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引用次数: 0

摘要

在这里，我们报告了一种方法，smol-seq（小分子测序），使用结构开关适体（SSAs）和DNA测序来量化代谢物。在smol-seq中，每个SSA检测单个目标分子并在目标结合上释放独特的DNA条形码。因此，对释放的条形码进行测序可以读出代谢物水平。我们发现ssa具有高度特异性，并且可以复用以并行检测多个靶标，从而将DNA测序的力量带入代谢组学。

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Quantifying metabolites using structure-switching aptamers coupled to DNA sequencing

Here we report a method, smol-seq (small-molecule sequencing), using structure-switching aptamers (SSAs) and DNA sequencing to quantify metabolites. In smol-seq, each SSA detects a single target molecule and releases a unique DNA barcode on target binding. Sequencing the released barcodes can, thus, read out metabolite levels. We show that SSAs are highly specific and can be multiplexed to detect multiple targets in parallel, bringing the power of DNA sequencing to metabolomics. Metabolites can be quantified using a combination of aptamers and DNA barcodes.

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来源期刊

Nature biotechnology 工程技术-生物工程与应用微生物

CiteScore

63.00

自引率

1.70%

发文量

382

审稿时长

3 months

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