Physicochemical properties of protein-polysaccharide-polyphenol bilayer composite film prepared by electrospinning layer-by-layer assembly technology

In this study, zein-wheat gluten composite fiber film (Z-G) and chitosan-gelatin-thymol composite fiber film (CS-GE-T) were physically assembled by electrospinning layer-by-layer assembly technology to prepare zein-wheat gluten / chitosan-gelatin-thymol double-layer composite fiber film (Z-G-C-T). Z-G and CS-GE-T were prepared by electrospinning, By comparing the functional properties of CS-GE-T with different concentrations of thymol, the optimal concentration ratio was selected as the second layer film (CS-GE-T 0.05); the bilayer composite fiber film was prepared by electrospinning layer-by-layer assembly technology. The thermal stability, mechanical properties, water vapor permeability and antibacterial properties of the double-layer composite fiber film are better than those of the single-layer composite fiber film. Its thermal stability was 14.74 % higher than that of the optimal ratio of double protein film, and 4.37 % higher than that of the optimal concentration CS-GE-T. The tensile strength, fracture strain and elastic modulus were increased by 2.07 MPa, 0.20 % and 141.50 MPa, respectively, compared with the optimal ratio of double protein composite fiber film. Through research, it was found that Z-G-C-T can be applied in the field of jujube fruit preservation, extending the shelf life of winter jujube, which provides new ideas for the field of multi-functional green preservation.