{"title":"一种用于EpCAM抗原检测的硫化铜掺杂2d - mxene超灵敏无标记电化学免疫传感器","authors":"Sweety, Saroj Paneru, Devendra Kumar","doi":"10.1002/aoc.7975","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Herein, a novel CuS-anchored 2D-Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> composite material was synthesized by a one-pot hydrothermal method. This CuS/Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> hybrid was used to prepare a novel label-free electrochemical immunosensor for EpCAM antigen detection. The immunosensor has been fabricated by electrophoretic deposition of CuS/Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> hybrid onto the ITO electrode followed by the immobilization of anti-EpCAM. The electrochemical biosensing studies of this fabricated immunosensor, BSA/anti-EpCAM/CuS/Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>@ITO demonstrated a wide linear range (0.01 fg/mL to 100 ng/mL) and low LOD (0.0057 fg/mL), together with high sensitivity (461.85 μA fg<sup>−1</sup> mL cm<sup>−2</sup>) for EpCAM antigen detection. The clinical validation of the proposed immunosensor was conducted with serum samples. Moreover, the immunosensor was found to be reproducible, specific, selective, and stable over a long period of 42 days.</p>\n </div>","PeriodicalId":8344,"journal":{"name":"Applied Organometallic Chemistry","volume":"39 2","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Copper Sulfide Doped 2D-MXene-Based Ultrasensitive Label-Free Electrochemical Immunosensor for EpCAM Antigen Detection\",\"authors\":\"Sweety, Saroj Paneru, Devendra Kumar\",\"doi\":\"10.1002/aoc.7975\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Herein, a novel CuS-anchored 2D-Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> composite material was synthesized by a one-pot hydrothermal method. This CuS/Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> hybrid was used to prepare a novel label-free electrochemical immunosensor for EpCAM antigen detection. The immunosensor has been fabricated by electrophoretic deposition of CuS/Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> hybrid onto the ITO electrode followed by the immobilization of anti-EpCAM. The electrochemical biosensing studies of this fabricated immunosensor, BSA/anti-EpCAM/CuS/Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>@ITO demonstrated a wide linear range (0.01 fg/mL to 100 ng/mL) and low LOD (0.0057 fg/mL), together with high sensitivity (461.85 μA fg<sup>−1</sup> mL cm<sup>−2</sup>) for EpCAM antigen detection. The clinical validation of the proposed immunosensor was conducted with serum samples. Moreover, the immunosensor was found to be reproducible, specific, selective, and stable over a long period of 42 days.</p>\\n </div>\",\"PeriodicalId\":8344,\"journal\":{\"name\":\"Applied Organometallic Chemistry\",\"volume\":\"39 2\",\"pages\":\"\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2025-01-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Organometallic Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/aoc.7975\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Organometallic Chemistry","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/aoc.7975","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
A Copper Sulfide Doped 2D-MXene-Based Ultrasensitive Label-Free Electrochemical Immunosensor for EpCAM Antigen Detection
Herein, a novel CuS-anchored 2D-Ti3C2Tx composite material was synthesized by a one-pot hydrothermal method. This CuS/Ti3C2Tx hybrid was used to prepare a novel label-free electrochemical immunosensor for EpCAM antigen detection. The immunosensor has been fabricated by electrophoretic deposition of CuS/Ti3C2Tx hybrid onto the ITO electrode followed by the immobilization of anti-EpCAM. The electrochemical biosensing studies of this fabricated immunosensor, BSA/anti-EpCAM/CuS/Ti3C2Tx@ITO demonstrated a wide linear range (0.01 fg/mL to 100 ng/mL) and low LOD (0.0057 fg/mL), together with high sensitivity (461.85 μA fg−1 mL cm−2) for EpCAM antigen detection. The clinical validation of the proposed immunosensor was conducted with serum samples. Moreover, the immunosensor was found to be reproducible, specific, selective, and stable over a long period of 42 days.
期刊介绍：
All new compounds should be satisfactorily identified and proof of their structure given according to generally accepted standards. Structural reports, such as papers exclusively dealing with synthesis and characterization, analytical techniques, or X-ray diffraction studies of metal-organic or organometallic compounds will not be considered. The editors reserve the right to refuse without peer review any manuscript that does not comply with the aims and scope of the journal. Applied Organometallic Chemistry publishes Full Papers, Reviews, Mini Reviews and Communications of scientific research in all areas of organometallic and metal-organic chemistry involving main group metals, transition metals, lanthanides and actinides. All contributions should contain an explicit application of novel compounds, for instance in materials science, nano science, catalysis, chemical vapour deposition, metal-mediated organic synthesis, polymers, bio-organometallics, metallo-therapy, metallo-diagnostics and medicine. Reviews of books covering aspects of the fields of focus are also published.
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