Aarón I. Vélez-Ramírez, Juan de Dios Moreno, Uriel G. Pérez-Guerrero, Antonio M. Juarez, Hector Castillo-Arriaga, Josefina Vázquez-Medrano, Ilane Hernández-Morales
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An open-source LED lamp for use with the LI-6800 photosynthesis system
Premise
Controlling light flux density during carbon dioxide assimilation measurements is essential in photosynthesis research. Commercial lamps are expensive and are based on monochromatic light-emitting diodes (LEDs), which deviate significantly in their spectral distribution compared to sunlight.
Methods and Results
Using LED-emitted white light with a color temperature similar to sunlight, we developed a cost-effective lamp compatible with the LI-6800 Portable Photosynthesis System. When coupled with customized software, the lamp can be controlled via the LI-6800 console by a user or Python scripts. Testing and calibration show that the lamp meets the quality needed to estimate photosynthesis parameters.
Conclusions
The lamp can be built using a basic electronics lab and a 3D printer. Calibration instructions are supplied and only require equipment commonly available at plant science laboratories. The lamp is a cost-effective alternative to perform photosynthesis research coupled with the popular LI-6800 photosynthesis measuring system.
期刊介绍：
Applications in Plant Sciences (APPS) is a monthly, peer-reviewed, open access journal promoting the rapid dissemination of newly developed, innovative tools and protocols in all areas of the plant sciences, including genetics, structure, function, development, evolution, systematics, and ecology. Given the rapid progress today in technology and its application in the plant sciences, the goal of APPS is to foster communication within the plant science community to advance scientific research. APPS is a publication of the Botanical Society of America, originating in 2009 as the American Journal of Botany''s online-only section, AJB Primer Notes & Protocols in the Plant Sciences.
APPS publishes the following types of articles: (1) Protocol Notes describe new methods and technological advancements; (2) Genomic Resources Articles characterize the development and demonstrate the usefulness of newly developed genomic resources, including transcriptomes; (3) Software Notes detail new software applications; (4) Application Articles illustrate the application of a new protocol, method, or software application within the context of a larger study; (5) Review Articles evaluate available techniques, methods, or protocols; (6) Primer Notes report novel genetic markers with evidence of wide applicability.
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