Xiaofang Zheng, Weiheng Kong, Xiaoxia Dai, Changjun You
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引用次数: 0
摘要
8-氧鸟嘌呤（8-oxoG）不仅是氧化 DNA 损伤的生物标志物，还是哺乳动物细胞中的表观遗传类似调节因子。鉴定和描述 8-oxoG 结合蛋白对于进一步了解 8-oxoG 的生物学后果至关重要。在这里，我们通过定量蛋白质组学方法鉴定出人类 Y-box 结合蛋白 1（YBX1）是 DNA 中 8-oxoG 修饰的新型结合蛋白。此外，我们还发现，缺乏YBX1会大大降低细胞对氧化应激的敏感性，并促进嵌入DNA中的8-oxoG的修复。这些发现为了解YBX1与DNA中8-oxoG修饰之间功能相互作用的生物学意义提供了新的视角。
YBX1 Modulates 8-Oxoguanine Recognition and Repair in DNA.
8-Oxoguanine (8-oxoG) is not only a biomarker of oxidative DNA damage but also an epigenetic-like regulator in mammalian cells. The identification and characterization of 8-oxoG-binding proteins would be crucial for further understanding the biological consequences of 8-oxoG. Here, we identified human Y-box-binding protein 1 (YBX1) as a novel binding protein for 8-oxoG modification in DNA by using a quantitative proteomic approach. Moreover, we found that the deficiency of YBX1 can substantially decrease the cellular sensitivity to oxidative stress and facilitate the repair of 8-oxoG embedded in DNA. These findings provided new insight into the biological significance of the functional interplay between YBX1 and 8-oxoG modification in DNA.
期刊介绍：
ACS Chemical Biology provides an international forum for the rapid communication of research that broadly embraces the interface between chemistry and biology.
The journal also serves as a forum to facilitate the communication between biologists and chemists that will translate into new research opportunities and discoveries. Results will be published in which molecular reasoning has been used to probe questions through in vitro investigations, cell biological methods, or organismic studies.
We welcome mechanistic studies on proteins, nucleic acids, sugars, lipids, and nonbiological polymers. The journal serves a large scientific community, exploring cellular function from both chemical and biological perspectives. It is understood that submitted work is based upon original results and has not been published previously.
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