IF 1.4 3区 医学Q4 BIOCHEMISTRY & MOLECULAR BIOLOGYMolecular VisionPub Date : 2024-10-10eCollection Date: 2024-01-01
Xue Han, Yaru Hu, Yue Chen, Jinbiao Cai, Yaru Chen, Na Li, Che Xu, Qi Zhou, Fengchao Wang, Jianfeng Wang
{"title":"病理性近视和单纯性高度近视白内障患者房水中细胞因子的表达。","authors":"Xue Han, Yaru Hu, Yue Chen, Jinbiao Cai, Yaru Chen, Na Li, Che Xu, Qi Zhou, Fengchao Wang, Jianfeng Wang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>To explore the role of cytokines during the progression process of cataract patients with pathologic myopia (PMC) and simple high myopia (SHMC).</p><p><strong>Methods: </strong>A total of 63 cataract patients who underwent cataract surgery were classified into a PMC group (22 eyes), an SHMC group (21 eyes), and an age-related cataract (ARC) group (20 eyes), based on axial length (AL) and International Myopia Institute (IMI)'s classification. Aqueous humor samples were extracted before surgery. Cytometric bead array (CBA) was employed to measure the level of interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), interleukin-8 (IL-8), transforming growth factor-β1 (TGF-β1), basic fibroblast growth factor (bFGF), interleukin-10 (IL-10), interleukin-17a (IL-17a), interleukin-1β (IL-1β), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor -α (TNF-α), intercellular adhesion molecule (ICAM), and vascular cell adhesion molecule (VCAM). Additionally, the correlations between cytokines and the AL or myopic maculopathy categories were examined.</p><p><strong>Results: </strong>VEGF, IL-6, MCP-1, ICAM, and VCAM (all p<0.001), TGF-β1 (p=0.018), and IL-8 (p=0.008) were statistically different among the three groups. In parallel, the levels of VCAM (<i>r</i>=0.718), MCP-1 (<i>r</i>=0.591), ICAM (<i>r</i>=0.584), IL-8 (<i>r</i>=0.435), IL-6 (<i>r</i>=0.396), and TNF-α (<i>r</i>=0.280) were positively associated with myopic maculopathy, while VEGF (<i>r</i>=-0.542), TGF-β1 (<i>r</i>=-0.381), and IL-17a (<i>r</i>=-0.284) were correlated inversely with myopic maculopathy (all p<0.05). Furthermore, a significant positive correlation was observed between AL and levels of VCAM (<i>r</i>=0.726), MCP-1 (<i>r</i>=0.644), ICAM (<i>r</i>=0.573), IL-6 (<i>r</i>=0.386), and IL-8(<i>r</i>=0.376). VEGF (<i>r</i>=-0.610), TGF-β1 (<i>r</i>=-0.361), and IL-17a (<i>r</i>=-0.319) were inversely associated with AL (all p<0.05). Further analysis using multiple regression indicated that, after adjusting for confounding factors, lower VEGF and higher VCAM were significantly associated with AL. However, the limitations of this study were reflected in the inability to determine whether the changes in cytokines were the consequences or causes of the formation of high myopia.</p><p><strong>Conclusions: </strong>The pathogeneses of PMC and SHMC may differ, and there are significant changes associated with inflammation and the immune response in eyes with PMC.</p>","PeriodicalId":18866,"journal":{"name":"Molecular Vision","volume":"30 ","pages":"369-377"},"PeriodicalIF":1.4000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829778/pdf/","citationCount":"0","resultStr":"{\"title\":\"Expression of cytokines in the aqueous humor of cataract patients with pathologic myopia and simple high myopia.\",\"authors\":\"Xue Han, Yaru Hu, Yue Chen, Jinbiao Cai, Yaru Chen, Na Li, Che Xu, Qi Zhou, Fengchao Wang, Jianfeng Wang\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>To explore the role of cytokines during the progression process of cataract patients with pathologic myopia (PMC) and simple high myopia (SHMC).</p><p><strong>Methods: </strong>A total of 63 cataract patients who underwent cataract surgery were classified into a PMC group (22 eyes), an SHMC group (21 eyes), and an age-related cataract (ARC) group (20 eyes), based on axial length (AL) and International Myopia Institute (IMI)'s classification. Aqueous humor samples were extracted before surgery. Cytometric bead array (CBA) was employed to measure the level of interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), interleukin-8 (IL-8), transforming growth factor-β1 (TGF-β1), basic fibroblast growth factor (bFGF), interleukin-10 (IL-10), interleukin-17a (IL-17a), interleukin-1β (IL-1β), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor -α (TNF-α), intercellular adhesion molecule (ICAM), and vascular cell adhesion molecule (VCAM). Additionally, the correlations between cytokines and the AL or myopic maculopathy categories were examined.</p><p><strong>Results: </strong>VEGF, IL-6, MCP-1, ICAM, and VCAM (all p<0.001), TGF-β1 (p=0.018), and IL-8 (p=0.008) were statistically different among the three groups. In parallel, the levels of VCAM (<i>r</i>=0.718), MCP-1 (<i>r</i>=0.591), ICAM (<i>r</i>=0.584), IL-8 (<i>r</i>=0.435), IL-6 (<i>r</i>=0.396), and TNF-α (<i>r</i>=0.280) were positively associated with myopic maculopathy, while VEGF (<i>r</i>=-0.542), TGF-β1 (<i>r</i>=-0.381), and IL-17a (<i>r</i>=-0.284) were correlated inversely with myopic maculopathy (all p<0.05). Furthermore, a significant positive correlation was observed between AL and levels of VCAM (<i>r</i>=0.726), MCP-1 (<i>r</i>=0.644), ICAM (<i>r</i>=0.573), IL-6 (<i>r</i>=0.386), and IL-8(<i>r</i>=0.376). VEGF (<i>r</i>=-0.610), TGF-β1 (<i>r</i>=-0.361), and IL-17a (<i>r</i>=-0.319) were inversely associated with AL (all p<0.05). Further analysis using multiple regression indicated that, after adjusting for confounding factors, lower VEGF and higher VCAM were significantly associated with AL. However, the limitations of this study were reflected in the inability to determine whether the changes in cytokines were the consequences or causes of the formation of high myopia.</p><p><strong>Conclusions: </strong>The pathogeneses of PMC and SHMC may differ, and there are significant changes associated with inflammation and the immune response in eyes with PMC.</p>\",\"PeriodicalId\":18866,\"journal\":{\"name\":\"Molecular Vision\",\"volume\":\"30 \",\"pages\":\"369-377\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829778/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Vision\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Vision","FirstCategoryId":"3","ListUrlMain":"","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Expression of cytokines in the aqueous humor of cataract patients with pathologic myopia and simple high myopia.
Purpose: To explore the role of cytokines during the progression process of cataract patients with pathologic myopia (PMC) and simple high myopia (SHMC).
Methods: A total of 63 cataract patients who underwent cataract surgery were classified into a PMC group (22 eyes), an SHMC group (21 eyes), and an age-related cataract (ARC) group (20 eyes), based on axial length (AL) and International Myopia Institute (IMI)'s classification. Aqueous humor samples were extracted before surgery. Cytometric bead array (CBA) was employed to measure the level of interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), interleukin-8 (IL-8), transforming growth factor-β1 (TGF-β1), basic fibroblast growth factor (bFGF), interleukin-10 (IL-10), interleukin-17a (IL-17a), interleukin-1β (IL-1β), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor -α (TNF-α), intercellular adhesion molecule (ICAM), and vascular cell adhesion molecule (VCAM). Additionally, the correlations between cytokines and the AL or myopic maculopathy categories were examined.
Results: VEGF, IL-6, MCP-1, ICAM, and VCAM (all p<0.001), TGF-β1 (p=0.018), and IL-8 (p=0.008) were statistically different among the three groups. In parallel, the levels of VCAM (r=0.718), MCP-1 (r=0.591), ICAM (r=0.584), IL-8 (r=0.435), IL-6 (r=0.396), and TNF-α (r=0.280) were positively associated with myopic maculopathy, while VEGF (r=-0.542), TGF-β1 (r=-0.381), and IL-17a (r=-0.284) were correlated inversely with myopic maculopathy (all p<0.05). Furthermore, a significant positive correlation was observed between AL and levels of VCAM (r=0.726), MCP-1 (r=0.644), ICAM (r=0.573), IL-6 (r=0.386), and IL-8(r=0.376). VEGF (r=-0.610), TGF-β1 (r=-0.361), and IL-17a (r=-0.319) were inversely associated with AL (all p<0.05). Further analysis using multiple regression indicated that, after adjusting for confounding factors, lower VEGF and higher VCAM were significantly associated with AL. However, the limitations of this study were reflected in the inability to determine whether the changes in cytokines were the consequences or causes of the formation of high myopia.
Conclusions: The pathogeneses of PMC and SHMC may differ, and there are significant changes associated with inflammation and the immune response in eyes with PMC.
期刊介绍：
Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical).
Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints.
For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.
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