不同的非靶点机制是结核苋种群对麦草畏和2,4 - D产生抗性的基础

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2025-02-18 DOI:10.1002/ps.8712

Isabel Schlegel Werle, Lucas Kopecky Bobadilla, Damilola A Raiyemo, Alexander J Lopez, Filipi Mesquita Machado, Patrick J Tranel

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引用次数: 0

摘要

背景：damaranthus结核（Moq.）水麻是几种对合成生长素除草剂（SAHs）具有抗性的杂草之一。在SAHs的耐药机制中，非靶位耐药（NTSR）因其复杂性而受到特别关注。在此，我们将连锁图谱与转录组分析结合起来，探索了多重抗除草剂结核杆菌群体（CHR）中两种SAHs（麦草畏和2,4 - D）的NTSR机制。结果F2定位人群的表型评估表明，CHR中麦草畏和2,4 - D抗性均存在多基因基础。麦草畏和2,4 - D处理的表型反应之间存在弱相关性。连锁定位分析显示，与麦草畏和2,4 - D抗性相关的8个QTL区域被定位到7条结核分枝杆菌染色体上。总之，这些QTL区域分别解释了麦草畏和2,4 - D抗性表型变异的24.2%和23.1%。在两个抗性性状之间只发现了一个共定位的QTL区域。结论CHR种群对麦草畏和2,4 - D的抗性受多个位点的基因控制。抗性性状的弱表型和遗传关联表明，在该结核分枝杆菌种群中，不止一种NTSR机制赋予了麦草畏和2,4 - D抗性。©2025作者。《病虫害管理科学》由John Wiley &出版；我代表化学工业协会的儿子有限公司。

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Different nontarget-site mechanisms underlie resistance to dicamba and 2,4-D in an Amaranthus tuberculatus population

BACKGROUND

Amaranthus tuberculatus (Moq.) Sauer (waterhemp) has emerged as one of several weed species that is resistant to synthetic auxin herbicides (SAHs). Among the mechanisms of resistance to SAHs, nontarget-site resistance (NTSR) has been of particular concern owing to its complexity. Here, we integrated linkage mapping with transcriptome analysis to explore NTSR mechanisms to two SAHs, dicamba and 2,4-D, in a multiple-herbicide-resistant A. tuberculatus population (CHR).

RESULTS

Phenotypic evaluations of an F₂ mapping population indicated a polygenic basis for both dicamba and 2,4-D resistance in CHR. A weak correlation was observed between phenotypic responses to dicamba and 2,4-D treatments. Linkage mapping analyses revealed eight quantitative trait loci (QTL) regions associated with dicamba and 2,4-D resistance mapped to seven A. tuberculatus chromosomes. Together, these QTL regions explained 24.2 and 23.1% of the variation in dicamba- and 2,4-D-resistant phenotypes, respectively. Only one co-localized QTL region was found between the two resistance traits.

CONCLUSION

The results of this study demonstrated that resistance to dicamba and 2,4-D in the CHR population is under the control of genes at multiple loci. The weak phenotypic and genetic associations of resistance traits indicate that more than one NTSR mechanism confers resistance to dicamba and 2,4-D in this A. tuberculatus population. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.