Yang Zhou , Zuying Zhou , Lin Jin , Yue Peng , Jingwen Tang , Aimin Wang , Meng Zhou , Yueting Li , Lin Zheng , Yong Huang
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However, its mechanism regarding bacterial adhesion, remain unclear.</div></div><div><h3>Purpose</h3><div>To investigate the effects and mechanisms of action of aqueous <em>P. capitata</em> extracts (PCE) on UPEC adhesion in T24 cells and rat models.</div></div><div><h3>Methods</h3><div>Broth microdilution and growth experiments were used to explore the direct antibacterial effects of PCE on UPEC. Additionally, motility assays were conducted. Different microscopy methods were used to further examine the mechanisms of action. Transcriptomic analysis and RT-qPCR were used to explore mechanisms on a molecular level. Relevant molecules were assessed using western blotting and immunohistochemistry.</div></div><div><h3>Results</h3><div>PCE modulated UPEC motility by disrupting the fimbriae and flagella. UPEC pathways, including those essential for constructing fimbriae and flagella, and bacterial motility, were affected. PCE reduced UPEC adhesion and invasion of T24 cells, altering the protein expression of adhesion-related molecules, by modulating the secretion of extracellular vesicles (EVs). It improved blood and urine parameters, reduced inflammatory markers, and ameliorated pathological changes in the kidneys and bladder of rats. Furthermore, the expression of adhesion-related molecules in bladder tissues decreased in the UTI rat model.</div></div><div><h3>Conclusions</h3><div>This study provides new insights into the mechanisms of herbal medicines in treating UTIs.</div></div>","PeriodicalId":20212,"journal":{"name":"Phytomedicine","volume":"139 ","pages":"Article 156515"},"PeriodicalIF":11.3000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The effects and mechanisms of aqueous Persicaria capitata extract on uropathogenic Escherichia coli adhesion\",\"authors\":\"Yang Zhou , Zuying Zhou , Lin Jin , Yue Peng , Jingwen Tang , Aimin Wang , Meng Zhou , Yueting Li , Lin Zheng , Yong Huang\",\"doi\":\"10.1016/j.phymed.2025.156515\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Urinary tract infections (UTIs) in humans are common, with uropathogenic <em>Escherichia coli</em> (UPEC) being the primary pathogen. 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引用次数: 0
摘要
人类尿路感染（uti）很常见，尿路致病性大肠杆菌（UPEC）是主要病原体。UPEC的粘附能力是致病的重要因素。由于一线抗菌素的限制，猪皮桃（Persicaria capitata, buchh .- ham .）一种传统的中草药，常用于治疗各种泌尿系统疾病。然而，其与细菌粘附的机制尚不清楚。目的探讨花楸水提物（PCE）对T24细胞和大鼠UPEC黏附的影响及其作用机制。方法采用微量肉汤稀释和生长实验，探讨PCE对UPEC的直接抑菌作用。此外，还进行了运动测定。使用不同的显微镜方法进一步研究其作用机制。利用转录组学分析和RT-qPCR在分子水平上探索其机制。western blotting和免疫组织化学检测相关分子。结果spce通过破坏毛和鞭毛来调节upc的运动。UPEC途径，包括那些必不可少的构建毛和鞭毛，和细菌的运动，受到影响。PCE通过调节细胞外囊泡（EVs）的分泌，降低UPEC对T24细胞的粘附和侵袭，改变粘附相关分子的蛋白表达。它改善了大鼠的血液和尿液参数，减少了炎症标志物，改善了肾脏和膀胱的病理变化。此外，尿路感染大鼠模型膀胱组织中黏附相关分子的表达减少。结论本研究为中药治疗尿路感染的机制提供了新的思路。
The effects and mechanisms of aqueous Persicaria capitata extract on uropathogenic Escherichia coli adhesion
Background
Urinary tract infections (UTIs) in humans are common, with uropathogenic Escherichia coli (UPEC) being the primary pathogen. The adhesive capabilities of UPEC are a substantial pathogenicity factor. Due to limitations of first-line antibiotics, Persicaria capitata (Buch.-Ham. ex D. Don) H. Gross, a traditional Chinese medicinal plant, is frequently used to treat various urological disorders. However, its mechanism regarding bacterial adhesion, remain unclear.
Purpose
To investigate the effects and mechanisms of action of aqueous P. capitata extracts (PCE) on UPEC adhesion in T24 cells and rat models.
Methods
Broth microdilution and growth experiments were used to explore the direct antibacterial effects of PCE on UPEC. Additionally, motility assays were conducted. Different microscopy methods were used to further examine the mechanisms of action. Transcriptomic analysis and RT-qPCR were used to explore mechanisms on a molecular level. Relevant molecules were assessed using western blotting and immunohistochemistry.
Results
PCE modulated UPEC motility by disrupting the fimbriae and flagella. UPEC pathways, including those essential for constructing fimbriae and flagella, and bacterial motility, were affected. PCE reduced UPEC adhesion and invasion of T24 cells, altering the protein expression of adhesion-related molecules, by modulating the secretion of extracellular vesicles (EVs). It improved blood and urine parameters, reduced inflammatory markers, and ameliorated pathological changes in the kidneys and bladder of rats. Furthermore, the expression of adhesion-related molecules in bladder tissues decreased in the UTI rat model.
Conclusions
This study provides new insights into the mechanisms of herbal medicines in treating UTIs.
期刊介绍：
Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.
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