IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS STAR Protocols Pub Date : 2025-02-20 DOI:10.1016/j.xpro.2025.103647
Nityanand Bolshette, Hanna Bueno-Levy, Gad Asher
{"title":"Protocol for monitoring intrapulmonary vasodilation in mice using contrast-enhanced echocardiography.","authors":"Nityanand Bolshette, Hanna Bueno-Levy, Gad Asher","doi":"10.1016/j.xpro.2025.103647","DOIUrl":null,"url":null,"abstract":"<p><p>Monitoring intrapulmonary vasodilation in animal models is vital for understanding pulmonary vascular physiology and disease mechanisms. Here, we present a protocol for the real-time visualization and quantification of pulmonary microvascular dynamics in living mice using contrast echocardiography alongside microbubble injection. We describe steps for mouse preparation, baseline echocardiography, microbubble administration, and imaging to capture dynamic changes in pulmonary vasculature. Closely resembling clinical technique used in humans, this methodology provides an effective tool for investigating the pathophysiology of diverse cardio-pulmonary vascular diseases. For complete details on the use and execution of this protocol, please refer to Dandavate et al.<sup>1</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"6 1","pages":"103647"},"PeriodicalIF":1.3000,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889970/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"STAR Protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.xpro.2025.103647","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

摘要

在动物模型中监测肺内血管扩张对了解肺血管生理和疾病机制至关重要。在此,我们介绍一种利用对比超声心动图和微泡注射对活体小鼠的肺微血管动态进行实时可视化和量化的方案。我们描述了小鼠准备、基线超声心动图、微泡注射和成像等步骤,以捕捉肺血管的动态变化。这种方法与用于人类的临床技术非常相似,是研究各种心肺血管疾病病理生理学的有效工具。有关该方案使用和执行的完整细节,请参阅 Dandavate 等人的文章1。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Protocol for monitoring intrapulmonary vasodilation in mice using contrast-enhanced echocardiography.

Monitoring intrapulmonary vasodilation in animal models is vital for understanding pulmonary vascular physiology and disease mechanisms. Here, we present a protocol for the real-time visualization and quantification of pulmonary microvascular dynamics in living mice using contrast echocardiography alongside microbubble injection. We describe steps for mouse preparation, baseline echocardiography, microbubble administration, and imaging to capture dynamic changes in pulmonary vasculature. Closely resembling clinical technique used in humans, this methodology provides an effective tool for investigating the pathophysiology of diverse cardio-pulmonary vascular diseases. For complete details on the use and execution of this protocol, please refer to Dandavate et al.1.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
STAR Protocols
STAR Protocols Biochemistry, Genetics and Molecular Biology-General Biochemistry, Genetics and Molecular Biology
CiteScore
2.00
自引率
0.00%
发文量
789
审稿时长
10 weeks
期刊介绍:
期刊最新文献
Protocol for generating human cerebral organoids from two-dimensional cultures of pluripotent stem cells bypassing embryoid body aggregation. Protocol for cell-type-specific single-cell labeling and manipulation in Drosophila using a sparse driver system. Protocol for isolation, fractionation, and system biology-based profiling of gastrointestinal digested dairy colostrum and milk proteome. Protocol for isolating the single bacteriocyte from whiteflies for single-cell RNA-seq analysis. Protocol for simultaneous detection of PRRS virus infection and dying cell populations by a flow cytometry double-staining approach.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1