IF 3.6 3区 生物学 Q1 BIOLOGY Biology-Basel Pub Date : 2025-02-08 DOI:10.3390/biology14020173
Chengwu Zou, Fanglian He, Huinan Li, Lili Liu, Zuyang Qiu, Weiqing Dong
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引用次数: 0

摘要

Colocasia esculenta 是一种富含营养价值和淀粉的块茎作物,其全球意义促使人们进一步研究其块茎的发育:背景:以往的研究主要关注块茎内的淀粉积累,但对球茎膨大的基因和蛋白质组学基础的研究在很大程度上仍是空白。本研究旨在阐明参与这一过程的关键基因和蛋白质:方法:我们选取了'荔浦芋 1 号',在球茎发育的三个不同阶段进行了纵向淀粉含量分析、全长转录组测序和蛋白质组分析:结果:我们的研究结果表明,淀粉和支链淀粉含量随着茎秆的发育而显著增加,这表明淀粉的生物合成受时间调控。转录组和蛋白质组数据的整合确定了与淀粉和蔗糖代谢以及植物激素信号转导相关的差异表达基因和蛋白质:这项研究描述了对淀粉合成至关重要的时序基因表达模式,深入揭示了控制茎秆膨大和淀粉沉积的调控机制,为未来提高芋头产量和质量的分子育种策略提供了有价值的参考。
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Integrated Transcriptome and Proteome Analysis Provides New Insights into Starch and Sucrose Metabolism and Regulation of Corm Expansion Process in Colocasia esculenta.

The global significance of Colocasia esculenta, a tuber crop rich in nutritional value and starch, prompts further investigation into its corm development.

Background: Previous studies have focused on starch accumulation within the tubers, yet the genetic and proteomic basis of corm expansion remains largely unexplored. This study aims to elucidate the key genes and proteins involved in this process.

Methods: We selected 'Lipu Taro No.1' and conducted a longitudinal starch content analysis, full-length transcriptome sequencing, and a proteomic analysis during three distinct stages of corm development.

Results: Our findings reveal a significant increase in both amylose and amylopectin contents as the corm develops, indicating the temporal regulation of starch biosynthesis. The integration of transcriptome and proteomic data identified differentially expressed genes and proteins associated with starch and sucrose metabolism, as well as plant hormone signal transduction.

Conclusions: This study delineates a temporal gene expression pattern that is crucial for starch synthesis and provides insights into the regulatory mechanisms controlling corm expansion and starch deposition, offering valuable references for future molecular breeding strategies to enhance taro yield and quality.

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来源期刊
Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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