{"title":"Integrated Transcriptome and Proteome Analysis Provides New Insights into Starch and Sucrose Metabolism and Regulation of Corm Expansion Process in <i>Colocasia esculenta</i>.","authors":"Chengwu Zou, Fanglian He, Huinan Li, Lili Liu, Zuyang Qiu, Weiqing Dong","doi":"10.3390/biology14020173","DOIUrl":null,"url":null,"abstract":"<p><p>The global significance of <i>Colocasia esculenta</i>, a tuber crop rich in nutritional value and starch, prompts further investigation into its corm development.</p><p><strong>Background: </strong>Previous studies have focused on starch accumulation within the tubers, yet the genetic and proteomic basis of corm expansion remains largely unexplored. This study aims to elucidate the key genes and proteins involved in this process.</p><p><strong>Methods: </strong>We selected 'Lipu Taro No.1' and conducted a longitudinal starch content analysis, full-length transcriptome sequencing, and a proteomic analysis during three distinct stages of corm development.</p><p><strong>Results: </strong>Our findings reveal a significant increase in both amylose and amylopectin contents as the corm develops, indicating the temporal regulation of starch biosynthesis. The integration of transcriptome and proteomic data identified differentially expressed genes and proteins associated with starch and sucrose metabolism, as well as plant hormone signal transduction.</p><p><strong>Conclusions: </strong>This study delineates a temporal gene expression pattern that is crucial for starch synthesis and provides insights into the regulatory mechanisms controlling corm expansion and starch deposition, offering valuable references for future molecular breeding strategies to enhance taro yield and quality.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 2","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851817/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biology-Basel","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/biology14020173","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOLOGY","Score":null,"Total":0}
Integrated Transcriptome and Proteome Analysis Provides New Insights into Starch and Sucrose Metabolism and Regulation of Corm Expansion Process in Colocasia esculenta.
The global significance of Colocasia esculenta, a tuber crop rich in nutritional value and starch, prompts further investigation into its corm development.
Background: Previous studies have focused on starch accumulation within the tubers, yet the genetic and proteomic basis of corm expansion remains largely unexplored. This study aims to elucidate the key genes and proteins involved in this process.
Methods: We selected 'Lipu Taro No.1' and conducted a longitudinal starch content analysis, full-length transcriptome sequencing, and a proteomic analysis during three distinct stages of corm development.
Results: Our findings reveal a significant increase in both amylose and amylopectin contents as the corm develops, indicating the temporal regulation of starch biosynthesis. The integration of transcriptome and proteomic data identified differentially expressed genes and proteins associated with starch and sucrose metabolism, as well as plant hormone signal transduction.
Conclusions: This study delineates a temporal gene expression pattern that is crucial for starch synthesis and provides insights into the regulatory mechanisms controlling corm expansion and starch deposition, offering valuable references for future molecular breeding strategies to enhance taro yield and quality.
期刊介绍:
Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.