IF 3.6 3区 生物学 Q1 BIOLOGY Biology-Basel Pub Date : 2025-02-01 DOI:10.3390/biology14020149
Jessie Ong, H Craig Heller, Elsa Pittaras
{"title":"Selectively Blocking Small Conductance Ca<sup>2+</sup>-Activated K<sup>+</sup> Channels Improves Cognition in Aged Mice.","authors":"Jessie Ong, H Craig Heller, Elsa Pittaras","doi":"10.3390/biology14020149","DOIUrl":null,"url":null,"abstract":"<p><p>Aging is associated with decreased neuronal sensitivity and activity that creates deficits in cognitive processes, including learning, memory, motivation, general activity, and other behaviors. These effects are due in part to decreased intracellular Ca<sup>2+</sup> homeostasis, increasing hyperpolarization of the resting potential in aged neurons and therefore decreasing their excitability. To reduce hyperpolarization in aged mice, we used apamin, a selective small conductance Ca<sup>2+</sup>-activated K<sup>+</sup> (sK<sub>Ca</sub>) channel blocker. By blocking the sK<sub>Ca</sub> channels, apamin decreases the egress of the K<sup>+</sup> out of the cell, reducing its hyperpolarization and causing it to be closer to threshold potential. As a result, neurons should be more sensitive to excitatory stimuli and more active. We evaluated the performance of aged mice in a selection of cognitive and behavioral tests prior to and after systemic applications of apamin or the vehicle saline. Apamin improved performance in short-term memory, increased attention to tasks, and decreased anhedonia. Apamin had no significant effect on long-term spatial and recognition memory, risk-taking behavior, sociability, and anxiety. Our results are compatible with the known effects of sK<sub>Ca</sub> channel blockade on neuronal sensitivity and activity; however, these short-term effects were not reflected in longer-term alterations of neural plasticity responsible for long-term spatial and recognition memory or other more complex cognitive processes we evaluated.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 2","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851921/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biology-Basel","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/biology14020149","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

衰老与神经元敏感性和活性下降有关,这会导致认知过程出现缺陷,包括学习、记忆、动机、一般活动和其他行为。造成这些影响的部分原因是细胞内 Ca2+ 平衡下降,增加了老化神经元静息电位的超极化,从而降低了它们的兴奋性。为了降低老龄小鼠的超极化,我们使用了一种选择性小电导 Ca2+ 激活 K+(sKCa)通道阻断剂阿帕明。通过阻断 sKCa 通道,阿帕明可减少 K+从细胞中排出,从而降低细胞的超极化,使其更接近阈电位。因此,神经元应该对兴奋性刺激更敏感、更活跃。在全身应用阿帕明或生理盐水之前和之后,我们评估了老年小鼠在一系列认知和行为测试中的表现。阿帕明改善了小鼠的短期记忆能力,提高了小鼠对任务的注意力,并减轻了小鼠的失神症状。阿帕明对长期空间记忆和识别记忆、冒险行为、社交能力和焦虑没有明显影响。我们的研究结果与已知的 sKCa 通道阻断对神经元敏感性和活动的影响相符;但是,这些短期影响并没有反映在负责长期空间记忆和识别记忆或我们评估的其他更复杂的认知过程的神经可塑性的长期改变上。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Selectively Blocking Small Conductance Ca2+-Activated K+ Channels Improves Cognition in Aged Mice.

Aging is associated with decreased neuronal sensitivity and activity that creates deficits in cognitive processes, including learning, memory, motivation, general activity, and other behaviors. These effects are due in part to decreased intracellular Ca2+ homeostasis, increasing hyperpolarization of the resting potential in aged neurons and therefore decreasing their excitability. To reduce hyperpolarization in aged mice, we used apamin, a selective small conductance Ca2+-activated K+ (sKCa) channel blocker. By blocking the sKCa channels, apamin decreases the egress of the K+ out of the cell, reducing its hyperpolarization and causing it to be closer to threshold potential. As a result, neurons should be more sensitive to excitatory stimuli and more active. We evaluated the performance of aged mice in a selection of cognitive and behavioral tests prior to and after systemic applications of apamin or the vehicle saline. Apamin improved performance in short-term memory, increased attention to tasks, and decreased anhedonia. Apamin had no significant effect on long-term spatial and recognition memory, risk-taking behavior, sociability, and anxiety. Our results are compatible with the known effects of sKCa channel blockade on neuronal sensitivity and activity; however, these short-term effects were not reflected in longer-term alterations of neural plasticity responsible for long-term spatial and recognition memory or other more complex cognitive processes we evaluated.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
期刊最新文献
The Role of tRNA-Derived Small RNAs (tsRNAs) in Regulating Cell Death of Cardiovascular Diseases. Effect of Fertilization on the Performance of Adult Pinus pinea Trees. Long-Term Engraftment of Cryopreserved Human Neurons for In Vivo Disease Modeling in Neurodegenerative Disease. CNPY2 in Solid Tumors: Mechanisms, Biomarker Potential, and Therapeutic Implications. Characterization of Exosome-like Nanoparticles from Saffron Tepals and Their Immunostimulatory Activity.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1