Moran Hu , Hai Huang , Meng Jia , Min Xu , Malin Chen , Junxiang Wu , Shouyong Gu , Hongwei Liang , Hongwen Zhou , Yingyun Gong
{"title":"血清细胞外囊泡中的一组mirna可作为MASLD的新型诊断生物标志物。","authors":"Moran Hu , Hai Huang , Meng Jia , Min Xu , Malin Chen , Junxiang Wu , Shouyong Gu , Hongwei Liang , Hongwen Zhou , Yingyun Gong","doi":"10.1016/j.bj.2025.100838","DOIUrl":null,"url":null,"abstract":"<div><div>The rising global prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) poses a growing challenge to healthcare systems, stimulating substantial research efforts to develop reliable diagnostic methodologies. Emerging evidence highlights extracellular vesicles (EVs) as promising non-invasive biomarkers due to their roles in metabolic regulation and disease progression. This study investigated the diagnostic potential of serum EV-derived microRNAs (miRNAs) for MASLD detection and staging. We developed a novel diagnostic approach combining wheat germ agglutinin (WGA)-coupled magnetic beads for EV capture with RT-qPCR analysis, creating a streamlined two-step protocol that eliminates conventional purification requirements. MiR-574-3p, miR-542-3p, and miR-200a-3p in serum EVs were significantly elevated in patients with MASLD, indicating their potential as non-invasive biomarkers. Here, our established platform offers a clinically feasible solution for EVs isolation and quantitative miRNA analysis, presenting significant advantages in diagnostic efficiency and practical implementation.</div></div>","PeriodicalId":8934,"journal":{"name":"Biomedical Journal","volume":"48 6","pages":"Article 100838"},"PeriodicalIF":4.4000,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A panel of miRNAs in serum extracellular vesicles serves as novel diagnostic biomarkers for MASLD\",\"authors\":\"Moran Hu , Hai Huang , Meng Jia , Min Xu , Malin Chen , Junxiang Wu , Shouyong Gu , Hongwei Liang , Hongwen Zhou , Yingyun Gong\",\"doi\":\"10.1016/j.bj.2025.100838\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The rising global prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) poses a growing challenge to healthcare systems, stimulating substantial research efforts to develop reliable diagnostic methodologies. Emerging evidence highlights extracellular vesicles (EVs) as promising non-invasive biomarkers due to their roles in metabolic regulation and disease progression. This study investigated the diagnostic potential of serum EV-derived microRNAs (miRNAs) for MASLD detection and staging. We developed a novel diagnostic approach combining wheat germ agglutinin (WGA)-coupled magnetic beads for EV capture with RT-qPCR analysis, creating a streamlined two-step protocol that eliminates conventional purification requirements. MiR-574-3p, miR-542-3p, and miR-200a-3p in serum EVs were significantly elevated in patients with MASLD, indicating their potential as non-invasive biomarkers. Here, our established platform offers a clinically feasible solution for EVs isolation and quantitative miRNA analysis, presenting significant advantages in diagnostic efficiency and practical implementation.</div></div>\",\"PeriodicalId\":8934,\"journal\":{\"name\":\"Biomedical Journal\",\"volume\":\"48 6\",\"pages\":\"Article 100838\"},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2025-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical Journal\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2319417025000125\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/2/28 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Journal","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2319417025000125","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/28 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
A panel of miRNAs in serum extracellular vesicles serves as novel diagnostic biomarkers for MASLD
The rising global prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) poses a growing challenge to healthcare systems, stimulating substantial research efforts to develop reliable diagnostic methodologies. Emerging evidence highlights extracellular vesicles (EVs) as promising non-invasive biomarkers due to their roles in metabolic regulation and disease progression. This study investigated the diagnostic potential of serum EV-derived microRNAs (miRNAs) for MASLD detection and staging. We developed a novel diagnostic approach combining wheat germ agglutinin (WGA)-coupled magnetic beads for EV capture with RT-qPCR analysis, creating a streamlined two-step protocol that eliminates conventional purification requirements. MiR-574-3p, miR-542-3p, and miR-200a-3p in serum EVs were significantly elevated in patients with MASLD, indicating their potential as non-invasive biomarkers. Here, our established platform offers a clinically feasible solution for EVs isolation and quantitative miRNA analysis, presenting significant advantages in diagnostic efficiency and practical implementation.
期刊介绍：
Biomedical Journal publishes 6 peer-reviewed issues per year in all fields of clinical and biomedical sciences for an internationally diverse authorship. Unlike most open access journals, which are free to readers but not authors, Biomedical Journal does not charge for subscription, submission, processing or publication of manuscripts, nor for color reproduction of photographs.
Clinical studies, accounts of clinical trials, biomarker studies, and characterization of human pathogens are within the scope of the journal, as well as basic studies in model species such as Escherichia coli, Caenorhabditis elegans, Drosophila melanogaster, and Mus musculus revealing the function of molecules, cells, and tissues relevant for human health. However, articles on other species can be published if they contribute to our understanding of basic mechanisms of biology.
A highly-cited international editorial board assures timely publication of manuscripts. Reviews on recent progress in biomedical sciences are commissioned by the editors.
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