IF 3.3 3区 医学 Q3 IMMUNOLOGY Microbial pathogenesis Pub Date : 2025-02-28 DOI:10.1016/j.micpath.2025.107378
Khawla E. Alsamhary
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引用次数: 0

摘要

铜绿假单胞菌是一种可怕的机会性病原体,因其能够形成生物膜并产生有利于慢性感染的毒力因子而臭名昭著,尤其是在囊性纤维化患者中。消毒剂的滥用,加上环境泄漏和生物降解,导致微生物广泛接触亚致死浓度的消毒剂,特别是季铵化合物(QACs)。本研究调查了 QACs(特别是乙基苯烷基二甲基氯化铵(EBAC))与控制铜绿假单胞菌行为的法定量感应(QS)机制之间的相互作用。研究结果表明,暴露于亚最低抑制浓度(sub-MICs)的 EBAC 不仅能增强铜绿假单胞菌分离株的生物膜形成能力,还能调节关键 QS 调控基因的表达。值得注意的是,这些细菌显示出生物膜相关毒力因子(如脓氰蛋白和弹性蛋白酶)的产生增加,以及抗生素敏感性特征的改变,这表明它们正在向持续感染表型转变。这些研究结果表明,暴露于 QAC 可显著增加对抗生素和过氧化氢等外部应激源的耐药性。这些结果表明,有必要重新评估 QAC 在临床消毒环境中的功效,尤其是针对铜绿假单胞菌感染的功效,并强调了使用 QAC 可能会对细菌行为和毒力造成意想不到的后果。这项研究为QACs在调节QS介导的毒力和抗生素耐药性方面的作用提供了新的见解,为亚致死消毒剂暴露的相关风险提供了新的视角。
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The effect of quaternary ammonium compounds (QACs) on quorum sensing and resistance of P. aeruginosa in clinical settings
Pseudomonas aeruginosa, a formidable opportunistic pathogen, is notorious for its ability to form biofilms and produce virulence factors that favor chronic infections, especially in cystic fibrosis patients. The misuse of disinfectants, combined with environmental leakage and biodegradation, has led to widespread exposure of microorganisms to sub-lethal concentrations of disinfectants, particularly quaternary ammonium compounds (QACs). This study investigates the interaction between QACs, specifically ethylbenzalkyl dimethyl ammonium chloride (EBAC), and the quorum sensing (QS) mechanisms governing P. aeruginosa behavior. The results demonstrate that exposure to sub-minimum inhibitory concentrations (sub-MICs) of EBAC not only enhances the biofilm-forming capability of P. aeruginosa isolates but also modulates the expression of crucial QS-regulated genes. Notably, the bacteria exhibit increased production of biofilm-associated virulence factors such as pyocyanin and elastase, and altered antibiotic susceptibility profiles, indicating a shift towards persistent infection phenotypes. These findings reveal that QAC exposure can significantly increase resistance to antibiotics and external stressors like hydrogen peroxide. These results emphasize the need to reassess the efficacy of QACs in clinical disinfection settings, particularly against P. aeruginosa infections, and highlight the potential for unintended consequences of their use regarding bacterial behavior and virulence. This study provides novel insights into the role of QACs in modulating QS-mediated virulence and antibiotic resistance, offering a new perspective on the risks associated with sub-lethal disinfectant exposure.
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来源期刊
Microbial pathogenesis
Microbial pathogenesis 医学-免疫学
CiteScore
7.40
自引率
2.60%
发文量
472
审稿时长
56 days
期刊介绍: Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)
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