Siti Nur Ezzati Yazid, Jinap Selamat, Siti Izera Ismail, Maimunah Sanny, Nik Iskandar Putra Samsudin
{"title":"不同拮抗剂接种比例对玉米琼脂和玉米籽粒上黄曲霉毒素、赭曲霉毒素A和伏马菌素的拮抗效果。","authors":"Siti Nur Ezzati Yazid, Jinap Selamat, Siti Izera Ismail, Maimunah Sanny, Nik Iskandar Putra Samsudin","doi":"10.1093/jambio/lxaf049","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>The present work investigated the efficacy of native biocontrol candidates (antagonists) against aflatoxins, ochratoxin A (OTA), and fumonisins produced by native mycotoxigenic fungi isolated from Malaysia at different pathogen: antagonist inoculum ratios on grain corn agar and grain corn kernels.</p><p><strong>Methods and results: </strong>Five pathogen: antagonist inoculum ratios (100:0, 75:25, 50:50, 25:75, and 0:100) were employed. Non-aflatoxigenic Aspergillus flavus Af1KD and Af5TD, and Penicillium janthinellum were used against aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) by A. flavus. Against OTA by A. niger, and fumonisin B1 (FB1) and fumonisin B2 (FB2) by Fusarium verticillioides and F. proliferatum, respectively, the antagonists Trichoderma asperelloides, T. asperellum, and T. harzianum were used. Non-aflatoxigenic A. flavus Af1KD was the most effective against AFB1 and AFB2 at all tested ratios and substrates. All Trichoderma spp. were effective against OTA by A. niger on grain corn agar at all tested ratios. Trichoderma asperelloides and T. asperellum were effective against FB1 and FB2 produced by F. verticillioides at all tested ratios and substrates. Trichoderma asperelloides was effective against FB1 and FB2 by F. proliferatum at all tested ratios and substrates.</p><p><strong>Conclusion: </strong>The native biocontrol candidates were effective against mycotoxigenic fungi and mycotoxin production on grain corn agar and grain corn kernels, and could be developed into biocontrol agents.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Efficacy of fungal antagonists against aflatoxins, ochratoxin A, and fumonisins at different pathogen:antagonist inoculum ratios on grain corn agar and grain corn kernel.\",\"authors\":\"Siti Nur Ezzati Yazid, Jinap Selamat, Siti Izera Ismail, Maimunah Sanny, Nik Iskandar Putra Samsudin\",\"doi\":\"10.1093/jambio/lxaf049\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>The present work investigated the efficacy of native biocontrol candidates (antagonists) against aflatoxins, ochratoxin A (OTA), and fumonisins produced by native mycotoxigenic fungi isolated from Malaysia at different pathogen: antagonist inoculum ratios on grain corn agar and grain corn kernels.</p><p><strong>Methods and results: </strong>Five pathogen: antagonist inoculum ratios (100:0, 75:25, 50:50, 25:75, and 0:100) were employed. Non-aflatoxigenic Aspergillus flavus Af1KD and Af5TD, and Penicillium janthinellum were used against aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) by A. flavus. Against OTA by A. niger, and fumonisin B1 (FB1) and fumonisin B2 (FB2) by Fusarium verticillioides and F. proliferatum, respectively, the antagonists Trichoderma asperelloides, T. asperellum, and T. harzianum were used. Non-aflatoxigenic A. flavus Af1KD was the most effective against AFB1 and AFB2 at all tested ratios and substrates. All Trichoderma spp. were effective against OTA by A. niger on grain corn agar at all tested ratios. Trichoderma asperelloides and T. asperellum were effective against FB1 and FB2 produced by F. verticillioides at all tested ratios and substrates. Trichoderma asperelloides was effective against FB1 and FB2 by F. proliferatum at all tested ratios and substrates.</p><p><strong>Conclusion: </strong>The native biocontrol candidates were effective against mycotoxigenic fungi and mycotoxin production on grain corn agar and grain corn kernels, and could be developed into biocontrol agents.</p>\",\"PeriodicalId\":15036,\"journal\":{\"name\":\"Journal of Applied Microbiology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-03-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1093/jambio/lxaf049\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/jambio/lxaf049","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Efficacy of fungal antagonists against aflatoxins, ochratoxin A, and fumonisins at different pathogen:antagonist inoculum ratios on grain corn agar and grain corn kernel.
Aim: The present work investigated the efficacy of native biocontrol candidates (antagonists) against aflatoxins, ochratoxin A (OTA), and fumonisins produced by native mycotoxigenic fungi isolated from Malaysia at different pathogen: antagonist inoculum ratios on grain corn agar and grain corn kernels.
Methods and results: Five pathogen: antagonist inoculum ratios (100:0, 75:25, 50:50, 25:75, and 0:100) were employed. Non-aflatoxigenic Aspergillus flavus Af1KD and Af5TD, and Penicillium janthinellum were used against aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) by A. flavus. Against OTA by A. niger, and fumonisin B1 (FB1) and fumonisin B2 (FB2) by Fusarium verticillioides and F. proliferatum, respectively, the antagonists Trichoderma asperelloides, T. asperellum, and T. harzianum were used. Non-aflatoxigenic A. flavus Af1KD was the most effective against AFB1 and AFB2 at all tested ratios and substrates. All Trichoderma spp. were effective against OTA by A. niger on grain corn agar at all tested ratios. Trichoderma asperelloides and T. asperellum were effective against FB1 and FB2 produced by F. verticillioides at all tested ratios and substrates. Trichoderma asperelloides was effective against FB1 and FB2 by F. proliferatum at all tested ratios and substrates.
Conclusion: The native biocontrol candidates were effective against mycotoxigenic fungi and mycotoxin production on grain corn agar and grain corn kernels, and could be developed into biocontrol agents.
期刊介绍：
Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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