新一代测序在临床应用中的技术改进

IF 1.3 Q3 MEDICAL LABORATORY TECHNOLOGY Practical Laboratory Medicine Pub Date : 2025-04-01 Epub Date: 2025-03-08 DOI:10.1016/j.plabm.2025.e00465

Xiaolei Xie, Weiguo Yin, Shuxia Xuan, Fuguang Li

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摘要

新一代测序（NGS）技术目前广泛应用于临床实验室。本文介绍了一种对NGS进行技术改进的方法，提高了NGS检测的成功率。这项研究表明，DNA库浓度是最高的摩尔比为100:1的适配器DNA。自修订的接头连接方法可以有效提高DNA文库的成功率，特别是人工操作的成功率。此外，改进的汇集方法为不同的NGS项目纳入了不同的DNA片段大小，证明对医学实验室应用有益。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Technical improvement on next-generation sequencing in clinical application

The next-generation sequencing (NGS) technology is currently widely utilized in clinical laboratories. This paper describes a method of technical improvement on NGS, which increases the success rates of NGS detection. This study reveals that the DNA library concentration is the highest at a molar ratio of 100:1 for the adapter to DNA. The self-revised method for adaptor ligation can effectively improve the success rate of DNA library, particularly in manual operations. Additionally, the modified pooling method, which incorporates various DNA fragment sizes for different NGS projects, proves beneficial for medical laboratory applications.

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来源期刊

Practical Laboratory Medicine Health Professions-Radiological and Ultrasound Technology

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

7 weeks

期刊介绍： Practical Laboratory Medicine is a high-quality, peer-reviewed, international open-access journal publishing original research, new methods and critical evaluations, case reports and short papers in the fields of clinical chemistry and laboratory medicine. The objective of the journal is to provide practical information of immediate relevance to workers in clinical laboratories. The primary scope of the journal covers clinical chemistry, hematology, molecular biology and genetics relevant to laboratory medicine, microbiology, immunology, therapeutic drug monitoring and toxicology, laboratory management and informatics. We welcome papers which describe critical evaluations of biomarkers and their role in the diagnosis and treatment of clinically significant disease, validation of commercial and in-house IVD methods, method comparisons, interference reports, the development of new reagents and reference materials, reference range studies and regulatory compliance reports. Manuscripts describing the development of new methods applicable to laboratory medicine (including point-of-care testing) are particularly encouraged, even if preliminary or small scale.