Yan Li, Jiamei Tang, Yulan Ma, Yujuan Yan, Fangfang Cheng, Kun Wang
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PBMCs transcriptome sequencing was performed in two groups (5 cases in each group), and significantly differentially expressed genes (DEGs) were screened. Additionally, GO function enrichment, KEGG enrichment and GSEA analyses were performed. RT-PCR helped to detect the relative GBP5, NLRP3 and caspase-1 expressions in two groups (30 cases in each group) while the two groups' caspase-1, IL-1β and IL-18 in plasma levels were measured by ELISA. Thus, clinical and laboratory datas of 60 hospitalized children with IM were evaluated.</p><p><strong>Results: </strong>Transcriptome sequencing results showed that 171 DEGs were screened in the NLIG group, compared with the LIG. Among them, 154 DEGs were up-regulated, and 17 were down-regulated, respectively. KEGG and GSEA analyses showed that IM-associated liver injury is correlated with a NOD-like receptor signaling pathway. Statistically significant differences were observed in the white blood cell and lymphocyte counts, CD3<sup>+</sup>CD4<sup>+</sup> T cells, CD3<sup>+</sup>CD8<sup>+</sup>T cells, alanine aminotransferase (ALT), aspartate transaminase (AST), and lactate dehydrogenase (LDH) of the two groups (p < 0.05). Compared with NLIG, GBP5, NLRP3 and caspase-1 expressions in PBMCs, as well as the caspase-1, IL-1β and IL-18 in plasma levels, were significantly higher in LIG (p < 0.001). A correlation analysis revealed a positive correlation of GBP5 with LDH, ALT, AST, CD3<sup>+</sup>CD8<sup>+</sup>T cells and NLRP3 (p < 0.05).</p><p><strong>Conclusions: </strong>Our findings demonstrate that GBP5 contributes to liver injury in IM children through the NLRP3-dependent pathway.</p>","PeriodicalId":14511,"journal":{"name":"Italian Journal of Pediatrics","volume":"51 1","pages":"72"},"PeriodicalIF":3.1000,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905478/pdf/","citationCount":"0","resultStr":"{\"title\":\"Clinical significance and pathogenesis of GBP5 in infectious mononucleosis associated liver injury.\",\"authors\":\"Yan Li, Jiamei Tang, Yulan Ma, Yujuan Yan, Fangfang Cheng, Kun Wang\",\"doi\":\"10.1186/s13052-025-01907-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Infectious mononucleosis (IM) is a common disease in children; however, liver injury is its most common complication. 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引用次数: 0
摘要
背景：传染性单核细胞增多症（IM）是儿童常见病；然而，肝损伤是其最常见的并发症。然而，IM并发肝损伤的发病机制尚不清楚。因此，本研究旨在探讨im相关性肝损伤的潜在机制。方法：本研究在苏州大学儿童医院采集70例住院IM患儿外周血。将患者分为肝损伤组（LIG, n = 35）和非肝损伤组（NLIG, n = 35）。随后，分离pbmc和血浆。两组（每组5例）进行pbmc转录组测序，筛选显著差异表达基因（DEGs）。此外，还进行了GO功能富集、KEGG富集和GSEA分析。RT-PCR检测两组患者（每组30例）GBP5、NLRP3和caspase-1的相对表达，ELISA检测两组患者血浆中caspase-1、IL-1β和IL-18的水平。因此，我们对60例住院儿童的临床和实验室数据进行了评估。结果：转录组测序结果显示，与LIG组相比，NLIG组共筛选到171个deg。其中上调154个，下调17个。KEGG和GSEA分析显示，im相关性肝损伤与nod样受体信号通路相关。两组患者白细胞和淋巴细胞计数、CD3+CD4+ T细胞、CD3+CD8+T细胞、谷丙转氨酶（ALT）、天冬氨酸转氨酶（AST）、乳酸脱氢酶（LDH） （p +CD8+T细胞和NLRP3）差异均有统计学意义（p > 0.05）。结论：GBP5通过NLRP3依赖途径参与IM患儿肝损伤。
Clinical significance and pathogenesis of GBP5 in infectious mononucleosis associated liver injury.
Background: Infectious mononucleosis (IM) is a common disease in children; however, liver injury is its most common complication. However, the pathogenesis of IM complicated with liver injury is ambiguous. Thus, this study aimed to explore the potential mechanism of IM-associated liver injury.
Methods: This study was conducted at the Children's Hospital of Soochow University by collecting peripheral blood of 70 hospitalized children with IM. These patients were categorized into the liver injury (LIG, n = 35) and the non-liver injury groups (NLIG, n = 35), respectively. Subsequently, PBMCs and plasma were separated and obtained. PBMCs transcriptome sequencing was performed in two groups (5 cases in each group), and significantly differentially expressed genes (DEGs) were screened. Additionally, GO function enrichment, KEGG enrichment and GSEA analyses were performed. RT-PCR helped to detect the relative GBP5, NLRP3 and caspase-1 expressions in two groups (30 cases in each group) while the two groups' caspase-1, IL-1β and IL-18 in plasma levels were measured by ELISA. Thus, clinical and laboratory datas of 60 hospitalized children with IM were evaluated.
Results: Transcriptome sequencing results showed that 171 DEGs were screened in the NLIG group, compared with the LIG. Among them, 154 DEGs were up-regulated, and 17 were down-regulated, respectively. KEGG and GSEA analyses showed that IM-associated liver injury is correlated with a NOD-like receptor signaling pathway. Statistically significant differences were observed in the white blood cell and lymphocyte counts, CD3+CD4+ T cells, CD3+CD8+T cells, alanine aminotransferase (ALT), aspartate transaminase (AST), and lactate dehydrogenase (LDH) of the two groups (p < 0.05). Compared with NLIG, GBP5, NLRP3 and caspase-1 expressions in PBMCs, as well as the caspase-1, IL-1β and IL-18 in plasma levels, were significantly higher in LIG (p < 0.001). A correlation analysis revealed a positive correlation of GBP5 with LDH, ALT, AST, CD3+CD8+T cells and NLRP3 (p < 0.05).
Conclusions: Our findings demonstrate that GBP5 contributes to liver injury in IM children through the NLRP3-dependent pathway.
期刊介绍：
Italian Journal of Pediatrics is an open access peer-reviewed journal that includes all aspects of pediatric medicine. The journal also covers health service and public health research that addresses primary care issues.
The journal provides a high-quality forum for pediatricians and other healthcare professionals to report and discuss up-to-the-minute research and expert reviews in the field of pediatric medicine. The journal will continue to develop the range of articles published to enable this invaluable resource to stay at the forefront of the field.
Italian Journal of Pediatrics, which commenced in 1975 as Rivista Italiana di Pediatria, provides a high-quality forum for pediatricians and other healthcare professionals to report and discuss up-to-the-minute research and expert reviews in the field of pediatric medicine. The journal will continue to develop the range of articles published to enable this invaluable resource to stay at the forefront of the field.
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