Rosa González-Sastre, Raquel Coronel, Patricia Mateos-Martínez, Laura Maeso, Elena Llorente-Beneyto, Alfonso Luque, Berta Anta, Victoria López-Alonso, Isabel Liste
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引用次数: 0
摘要
人脑类器官（hCOs）为人类大脑发育和疾病的研究提供了一个极好的模型。在这里，我们提出了一种直接从二维（2D）多能干细胞（PSC）培养中获得hCOs的方案，避免了细胞分离和后胚状体（EB）聚集。我们描述了将2D培养物置于神经命运和随后发展hCOs的步骤。然后我们详细介绍了不同细胞类型的评估。有关本协议使用和执行的完整细节，请参阅González-Sastre et al.1。
Protocol for generating human cerebral organoids from two-dimensional cultures of pluripotent stem cells bypassing embryoid body aggregation.
Human cerebral organoids (hCOs) provide an excellent model for the study of human brain development and disease. Here, we present a protocol to obtain hCOs directly from two-dimensional (2D) pluripotent stem cell (PSC) cultures, avoiding cell dissociation and posterior embryoid body (EB) aggregation. We describe steps for subjecting 2D cultures to a neural fate and subsequently developing hCOs. We then detail the evaluation of different cellular types. For complete details on the use and execution of this protocol, please refer to González-Sastre et al.1.
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