Ziyuan Lin , Weihao Kong , Ziyu Yan , Yili Xu , Yingwei Zhou , Lvna Qin , Mengli Chen , Chaolan Zhang
{"title":"Insights into the response of biological nitrogen removal process to organophosphate esters and the adaptive mechanisms of microbial community","authors":"Ziyuan Lin , Weihao Kong , Ziyu Yan , Yili Xu , Yingwei Zhou , Lvna Qin , Mengli Chen , Chaolan Zhang","doi":"10.1016/j.jwpe.2025.107472","DOIUrl":null,"url":null,"abstract":"<div><div>The increasing organophosphate esters (OPEs) in wastewater have aroused great concerns, but their impacts on biological nitrogen removal process remained unclear. The effects of two ubiquitous OPEs, tris(2-chloroethyl) phosphate (TCEP) and tris(n-butyl) phosphate (TnBP), on biological nitrogen removal performance and microbial community were explored in activated sludge system. The results indicated that nitrogen removal efficiency remained stable (71.29%<span><math><mo>−</mo></math></span>75.65%) with OPEs increased. Nitrite oxidation and denitrification processes were inhibited by high TnBP and TCEP concentrations, respectively. OPE-tolerant denitrifying bacteria (e.g., <em>Azonexus</em>, <em>Methyloversatilis</em>) were enriched under OPEs exposure. The oriented community succession resulted in more important deterministic process in community assembly and more interactions between nitrogen removal bacteria. Molecular ecological networks analysis indicated that more cooperation of keystone and functional taxa enhanced community stability under OPEs stress. The nitrogen metabolic functions were stimulated with increasing OPEs concentration, and the function of xenobiotics degradation was upregulated as the OPEs degradation bacteria enriched, which would alleviate the negative impacts of OPEs on the nitrogen removal process. Furthermore, carbon metabolic and electron transfer functions were altered to alleviate OPEs stress and facilitate denitrification. This study would provide new ecological insights into the adaptive evolution of microbial community to emerging contaminants in biological nitrogen removal systems.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"72 ","pages":"Article 107472"},"PeriodicalIF":6.3000,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of water process engineering","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214714425005446","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
引用次数: 0
摘要
废水中的有机磷酸酯(OPEs)日益增多,引起了人们的极大关注,但它们对生物脱氮过程的影响仍不明确。本研究探讨了两种普遍存在的有机磷酸酯--磷酸三(2-氯乙基)酯(TCEP)和磷酸三(正丁基)酯(TnBP)--在活性污泥系统中对生物脱氮性能和微生物群落的影响。结果表明,随着 OPEs 的增加,脱氮效率保持稳定(71.29%-75.65%)。亚硝酸盐氧化和反硝化过程分别受到高浓度 TnBP 和 TCEP 的抑制。耐 OPE 的反硝化细菌(如 Azonexus、Methyloversatilis)在暴露于 OPEs 的情况下富集。定向群落演替导致群落组装过程中更重要的决定性过程和脱氮细菌之间更多的相互作用。分子生态网络分析表明,在 OPEs 胁迫下,关键类群和功能类群的更多合作增强了群落的稳定性。随着 OPEs 浓度的增加,氮代谢功能受到刺激,而随着 OPEs 降解菌的富集,异生 物降解功能得到提升,这将减轻 OPEs 对脱氮过程的负面影响。此外,碳代谢和电子传递功能也发生了改变,从而减轻了 OPEs 胁迫,促进了反硝化作用。这项研究将为生物脱氮系统中微生物群落对新兴污染物的适应性进化提供新的生态学见解。
Insights into the response of biological nitrogen removal process to organophosphate esters and the adaptive mechanisms of microbial community
The increasing organophosphate esters (OPEs) in wastewater have aroused great concerns, but their impacts on biological nitrogen removal process remained unclear. The effects of two ubiquitous OPEs, tris(2-chloroethyl) phosphate (TCEP) and tris(n-butyl) phosphate (TnBP), on biological nitrogen removal performance and microbial community were explored in activated sludge system. The results indicated that nitrogen removal efficiency remained stable (71.29%75.65%) with OPEs increased. Nitrite oxidation and denitrification processes were inhibited by high TnBP and TCEP concentrations, respectively. OPE-tolerant denitrifying bacteria (e.g., Azonexus, Methyloversatilis) were enriched under OPEs exposure. The oriented community succession resulted in more important deterministic process in community assembly and more interactions between nitrogen removal bacteria. Molecular ecological networks analysis indicated that more cooperation of keystone and functional taxa enhanced community stability under OPEs stress. The nitrogen metabolic functions were stimulated with increasing OPEs concentration, and the function of xenobiotics degradation was upregulated as the OPEs degradation bacteria enriched, which would alleviate the negative impacts of OPEs on the nitrogen removal process. Furthermore, carbon metabolic and electron transfer functions were altered to alleviate OPEs stress and facilitate denitrification. This study would provide new ecological insights into the adaptive evolution of microbial community to emerging contaminants in biological nitrogen removal systems.
期刊介绍:
The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies