{"title":"对紫外线敏感的表皮葡萄球菌突变体的鉴定","authors":"Heloisa Guillobel, Alvaro C. Leitão","doi":"10.1016/0167-8817(88)90002-8","DOIUrl":null,"url":null,"abstract":"<div><p>Five UV-sensitive mutants obtained by <em>N</em>-methyl-<em>N</em>′-nitro-<em>N</em>-nitrosoguanidine (MNNG) treatment of the <em>Staphyloccocus epidermidis</em> W5 strain were characterized phenotypically by assaying their UV- and MNNG-sensitivities, lysogenic inducibility, host-cell reactivation and Weigle reactivation capacities. The results were compared with those of well-characterized <em>Escherichia coli</em> strains, permitting the identification of: 2 mutants that behave as <em>Uvr<sup>−</sup> Umu<sup>−</sup></em>; 1 mutant that appears analogous to <em>Uvr<sup>−</sup></em>; 1 mutant that resembles <em>LexA<sup>−</sup></em> and 1 mutant that exhibits a <em>RecA<sup>−</sup></em> phenotype. The study of these mutants can contribute to the understanding of the repair mechanisms in <em>S. epidermidis</em>.</p></div>","PeriodicalId":100936,"journal":{"name":"Mutation Research/DNA Repair Reports","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0167-8817(88)90002-8","citationCount":"3","resultStr":"{\"title\":\"Characterization of Staphylococcus epidermidis mutants sensitive to ultraviolet radiation\",\"authors\":\"Heloisa Guillobel, Alvaro C. Leitão\",\"doi\":\"10.1016/0167-8817(88)90002-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Five UV-sensitive mutants obtained by <em>N</em>-methyl-<em>N</em>′-nitro-<em>N</em>-nitrosoguanidine (MNNG) treatment of the <em>Staphyloccocus epidermidis</em> W5 strain were characterized phenotypically by assaying their UV- and MNNG-sensitivities, lysogenic inducibility, host-cell reactivation and Weigle reactivation capacities. The results were compared with those of well-characterized <em>Escherichia coli</em> strains, permitting the identification of: 2 mutants that behave as <em>Uvr<sup>−</sup> Umu<sup>−</sup></em>; 1 mutant that appears analogous to <em>Uvr<sup>−</sup></em>; 1 mutant that resembles <em>LexA<sup>−</sup></em> and 1 mutant that exhibits a <em>RecA<sup>−</sup></em> phenotype. The study of these mutants can contribute to the understanding of the repair mechanisms in <em>S. epidermidis</em>.</p></div>\",\"PeriodicalId\":100936,\"journal\":{\"name\":\"Mutation Research/DNA Repair Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0167-8817(88)90002-8\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mutation Research/DNA Repair Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0167881788900028\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research/DNA Repair Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0167881788900028","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Characterization of Staphylococcus epidermidis mutants sensitive to ultraviolet radiation
Five UV-sensitive mutants obtained by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) treatment of the Staphyloccocus epidermidis W5 strain were characterized phenotypically by assaying their UV- and MNNG-sensitivities, lysogenic inducibility, host-cell reactivation and Weigle reactivation capacities. The results were compared with those of well-characterized Escherichia coli strains, permitting the identification of: 2 mutants that behave as Uvr− Umu−; 1 mutant that appears analogous to Uvr−; 1 mutant that resembles LexA− and 1 mutant that exhibits a RecA− phenotype. The study of these mutants can contribute to the understanding of the repair mechanisms in S. epidermidis.
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